Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3190554..3191208 Replicon chromosome
Accession NZ_CP076259
Organism Escherichia coli strain WY517-2

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag A7A20_RS15460 Protein ID WP_000244772.1
Coordinates 3190554..3190961 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag A7A20_RS15465 Protein ID WP_000354046.1
Coordinates 3190942..3191208 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A20_RS15440 (3186511) 3186511..3188244 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
A7A20_RS15445 (3188250) 3188250..3188960 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
A7A20_RS15450 (3188985) 3188985..3189881 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
A7A20_RS15455 (3189993) 3189993..3190514 + 522 WP_001055874.1 flavodoxin FldB -
A7A20_RS15460 (3190554) 3190554..3190961 - 408 WP_000244772.1 protein YgfX Toxin
A7A20_RS15465 (3190942) 3190942..3191208 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
A7A20_RS15470 (3191451) 3191451..3192431 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
A7A20_RS15475 (3192508) 3192508..3193167 - 660 WP_000250274.1 hemolysin III family protein -
A7A20_RS15480 (3193331) 3193331..3193642 - 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
A7A20_RS15485 (3193687) 3193687..3195120 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
A7A20_RS15490 (3195177) 3195177..3195920 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T205041 WP_000244772.1 NZ_CP076259:c3190961-3190554 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T205041 NZ_CP101372:2794625-2794909 [Salmonella enterica]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGGCTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGACACAGAAGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT205041 WP_000354046.1 NZ_CP076259:c3191208-3190942 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT205041 NZ_CP101372:2794387-2794635 [Salmonella enterica]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGCAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References