205040

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3054283..3054866 Replicon chromosome
Accession NZ_CP076259
Organism Escherichia coli strain WY517-2

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag A7A20_RS14845 Protein ID WP_000254745.1
Coordinates 3054283..3054618 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag A7A20_RS14850 Protein ID WP_000581937.1
Coordinates 3054618..3054866 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A20_RS14825 (3049752) 3049752..3050114 + 363 WP_000034929.1 hypothetical protein -
A7A20_RS14830 (3050170) 3050170..3051468 - 1299 WP_000036723.1 phosphopyruvate hydratase -
A7A20_RS14835 (3051556) 3051556..3053193 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
A7A20_RS14840 (3053421) 3053421..3054212 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
A7A20_RS14845 (3054283) 3054283..3054618 - 336 WP_000254745.1 endoribonuclease MazF Toxin
A7A20_RS14850 (3054618) 3054618..3054866 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
A7A20_RS14855 (3054944) 3054944..3057178 - 2235 WP_000226815.1 GTP pyrophosphokinase -
A7A20_RS14860 (3057226) 3057226..3058527 - 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T205040 WP_000254745.1 NZ_CP076259:c3054618-3054283 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T205040 NZ_CP101372:2452227-2452330 [Salmonella enterica]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT205040 WP_000581937.1 NZ_CP076259:c3054866-3054618 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT205040 NZ_CP101372:c2452332-2452187 [Salmonella enterica]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LMB4

References