Detailed information of TA system TA205039

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2731447..2732072
• Replicon: chromosome
• Accession: NZ_CP076259
• Organism: Escherichia coli strain WY517-2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: A7A20_RS13280
• Protein ID: WP_000911330.1
• Coordinates: 2731447..2731845 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: A7A20_RS13285
• Protein ID: WP_000450524.1
• Coordinates: 2731845..2732072 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A20_RS13260 (2727325)	2727325..2727525	+	201	WP_000383836.1	YpfN family protein	-
A7A20_RS13265 (2727635)	2727635..2728333	-	699	WP_000679823.1	esterase	-
A7A20_RS13270 (2728407)	2728407..2730422	-	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
A7A20_RS13275 (2730437)	2730437..2731300	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
A7A20_RS13280 (2731447)	2731447..2731845	-	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
A7A20_RS13285 (2731845)	2731845..2732072	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
A7A20_RS13290 (2732226)	2732226..2732939	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
A7A20_RS13295 (2733152)	2733152..2734186	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
A7A20_RS13300 (2734203)	2734203..2735081	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
A7A20_RS13305 (2735227)	2735227..2735799	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
A7A20_RS13310 (2735799)	2735799..2736269	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T205039 WP_000911330.1 NZ_CP076259:c2731845-2731447 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T205039 NZ_CP101372:c1371416-1370889 [Salmonella enterica]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCGGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTACTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT205039 WP_000450524.1 NZ_CP076259:c2732072-2731845 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT205039 NZ_CP101372:c1371703-1371413 [Salmonella enterica]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACATACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGCGATACTAAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33