Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 71616..72249 Replicon chromosome
Accession NC_002945
Organism Mycobacterium bovis AF2122/97

Toxin (Protein)


Gene name vapC Uniprot ID P9WFC0
Locus tag BQ2027_RS00370 Protein ID WP_003400580.1
Coordinates 71848..72249 (+) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A7U4BR76
Locus tag BQ2027_RS00365 Protein ID WP_011799070.1
Coordinates 71616..71855 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BQ2027_RS00350 (BQ2027_MB0064) 66913..68352 + 1440 WP_003901784.1 FAD-binding oxidoreductase -
BQ2027_RS00355 68408..68569 + 162 WP_003899796.1 hypothetical protein -
BQ2027_RS00360 (BQ2027_MB0065) 68610..71549 + 2940 WP_010950333.1 UPF0182 family protein -
BQ2027_RS00365 (BQ2027_MB0065A) 71616..71855 + 240 WP_011799070.1 hypothetical protein Antitoxin
BQ2027_RS00370 (BQ2027_MB0066) 71848..72249 + 402 WP_003400580.1 type II toxin-antitoxin system ribonuclease VapC1 Toxin
BQ2027_RS00375 (BQ2027_MB0067C) 72301..74538 - 2238 WP_003400583.1 NADP-dependent isocitrate dehydrogenase -
BQ2027_RS00380 (BQ2027_MB0068C) 74656..75225 - 570 WP_003400591.1 TetR/AcrR family transcriptional regulator -
BQ2027_RS00385 (BQ2027_MB0069) 75328..76239 + 912 WP_003899798.1 SDR family NAD(P)-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Pae0151-like, PIN domain

(36-124)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14339.44 Da        Isoelectric Point: 4.8887

>T20503 WP_003400580.1 NC_002945:71848-72249 [Mycobacterium tuberculosis variant bovis AF2122/97]
VDECVVDAAAVVDALAGKGASAIVLRGLLKESISNAPHLLDAEVGHALRRAVLSDEISEEQARAALDALPYLIDNRYPHS
PRLIEYTWQLRHNVTFYDALYVALATALDVPLLTGDSRLAAAPGLPCEIKLVR

Download         Length: 402 bp

>T20503 NC_002945:71848-72249 [Mycobacterium tuberculosis variant bovis AF2122/97]
GTGGATGAATGTGTAGTCGACGCGGCGGCCGTGGTTGACGCTCTCGCCGGCAAGGGCGCCAGCGCGATCGTTCTGCGCGG
TTTGCTCAAGGAGTCGATTTCTAACGCGCCGCATTTGCTGGACGCAGAGGTCGGACATGCACTCCGCCGCGCCGTGCTCA
GCGACGAAATCTCCGAAGAGCAGGCTCGCGCCGCGTTGGATGCCTTGCCTTATCTCATCGACAATCGTTACCCGCACAGC
CCACGACTGATCGAATACACATGGCAGCTAAGGCACAACGTCACGTTCTACGACGCCCTTTACGTCGCACTGGCCACCGC
ACTGGATGTCCCGCTGCTCACGGGCGACTCGCGGCTTGCGGCCGCGCCGGGCCTTCCGTGCGAAATCAAACTCGTTCGGT
GA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 8745.89 Da        Isoelectric Point: 8.5960

>AT20503 WP_011799070.1 NC_002945:71616-71855 [Mycobacterium tuberculosis variant bovis AF2122/97]
MATIQVRDLPEDVAETYRRRATAAGQSLQTYMRTKLIEGVRGRDKAEVIEILEQALASTASPGISRETIEASRRELRGG

Download         Length: 240 bp

>AT20503 NC_002945:71616-71855 [Mycobacterium tuberculosis variant bovis AF2122/97]
ATGGCTACCATTCAAGTTCGGGATTTGCCCGAAGATGTCGCCGAAACCTATCGACGGCGCGCCACCGCAGCGGGGCAGTC
GCTGCAGACGTATATGCGCACCAAGCTCATCGAAGGGGTGCGGGGCCGAGACAAGGCCGAGGTAATCGAGATCCTGGAAC
AGGCGCTCGCCAGCACTGCCAGCCCAGGCATCAGCCGGGAGACCATCGAGGCATCCCGGCGGGAGCTCAGGGGTGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BR82

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BR76

References