Detailed information of TA system TA205028

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1621660..1622298
• Replicon: chromosome
• Accession: NZ_CP076259
• Organism: Escherichia coli strain WY517-2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: A7A20_RS07870
• Protein ID: WP_000813794.1
• Coordinates: 1621660..1621836 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: A7A20_RS07875
• Protein ID: WP_001270286.1
• Coordinates: 1621882..1622298 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A20_RS07850 (1617279)	1617279..1618493	-	1215	WP_001349372.1	BenE family transporter YdcO	-
A7A20_RS07855 (1618546)	1618546..1619082	+	537	WP_000429141.1	DNA-binding transcriptional regulator SutR	-
A7A20_RS07860 (1619155)	1619155..1621116	+	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
A7A20_RS07865 (1621208)	1621208..1621438	-	231	WP_000494244.1	YncJ family protein	-
A7A20_RS07870 (1621660)	1621660..1621836	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
A7A20_RS07875 (1621882)	1621882..1622298	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
A7A20_RS07880 (1622377)	1622377..1623783	+	1407	WP_000760629.1	PLP-dependent aminotransferase family protein	-
A7A20_RS07885 (1624028)	1624028..1625173	+	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
A7A20_RS07890 (1625191)	1625191..1626204	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
A7A20_RS07895 (1626205)	1626205..1627146	+	942	WP_001251319.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1615974..1617239	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T205028 WP_000813794.1 NZ_CP076259:1621660-1621836 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T205028 NZ_CP101369:c4724627-4724316 [Salmonella enterica]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGATCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT205028 WP_001270286.1 NZ_CP076259:1621882-1622298 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT205028 NZ_CP101369:c4724315-4724025 [Salmonella enterica]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAACCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Toxin

• PDB: 6HPB