Detailed information of TA system TA205020

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 550179..551016
• Replicon: chromosome
• Accession: NZ_CP076259
• Organism: Escherichia coli strain WY517-2

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: A7A20_RS02620
• Protein ID: WP_000227784.1
• Coordinates: 550179..550721 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: A7A20_RS02625
• Protein ID: WP_001297137.1
• Coordinates: 550705..551016 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A20_RS02600 (545718)	545718..546629	-	912	WP_000705847.1	2-dehydropantoate 2-reductase	-
A7A20_RS02605 (546797)	546797..547288	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
A7A20_RS02610 (547416)	547416..548780	-	1365	WP_001000978.1	MFS transporter	-
A7A20_RS02615 (549188)	549188..550123	+	936	WP_001297127.1	tetratricopeptide repeat protein	-
A7A20_RS02620 (550179)	550179..550721	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
A7A20_RS02625 (550705)	550705..551016	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
A7A20_RS02630 (551201)	551201..552091	-	891	WP_000971336.1	heme o synthase	-
A7A20_RS02635 (552103)	552103..552432	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
A7A20_RS02640 (552432)	552432..553046	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
A7A20_RS02645 (553036)	553036..555027	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
A7A20_RS02650 (555049)	555049..555996	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T205020 WP_000227784.1 NZ_CP076259:c550721-550179 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T205020 NZ_CP101369:1995638-1995970 [Salmonella enterica]
ATGGACAGAGGGGAAATCTGGCTCGTTTCACTGGATCCGATTGCCGGCCATGAGCAAAGTGGAAAACGTCCGGTACTCAT
CGTATCGCAAGCATCGTTTAACAAGCTGACCCGACTACCCGTTATTGTTCCCGTCACCAGCGGCGGAAACTTTGCCCGCG
CAGCCGGTTTTACCGTCTCCCTGGACGGCGCGGGAACAAAAACAACGGGCGTTATTCGCTGCGACCAGCCCAGAACTATT
GATATGGGCGCCCGGAACGGTAAGCGGCTGGAACGTATACCCGACGCTGTTGTGAATGAAGTGCTGGCCCGACTGGAGGC
CATTTTGAGCTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT205020 WP_001297137.1 NZ_CP076259:c551016-550705 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT205020 NZ_CP101369:1995380-1995637 [Salmonella enterica]
ATGTATACCACTCGTCTGAAAAAGGTTGGCGGATCCGTCATGCTGGCTGTTCCTCCCGCCGTGCTGAAAACGCTGGGGCT
GTCGACGGACAGCGAAGTGGGCATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCACGTTATTCGC
TTGAGGAACTGCTGGCGCAGTGCGATCCGCACGCTGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATCCTGTAA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3