Detailed information of TA system TA205013

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 1287040..1287632
• Replicon: chromosome
• Accession: NZ_CP076257
• Organism: Xanthomonas translucens pv. graminis strain ART-Xtg29

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A1M4L1P2
• Locus tag: KM433_RS05770
• Protein ID: WP_009580424.1
• Coordinates: 1287336..1287632 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: KM433_RS05765
• Protein ID: WP_009580422.1
• Coordinates: 1287040..1287333 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KM433_RS05735 (KM433_05720)	1282733..1283143	+	411	WP_039955568.1	pilin	-
KM433_RS05740	1283805..1284110	-	306	WP_231103532.1	hypothetical protein	-
KM433_RS05745	1284111..1284362	-	252	WP_009580414.1	transposase	-
KM433_RS05750 (KM433_05730)	1284462..1284782	-	321	WP_009580416.1	DUF86 domain-containing protein	-
KM433_RS05755 (KM433_05735)	1284779..1285069	-	291	WP_009580418.1	nucleotidyltransferase family protein	-
KM433_RS05760 (KM433_05740)	1285276..1287012	+	1737	WP_009580420.1	type IV-A pilus assembly ATPase PilB	-
KM433_RS05765 (KM433_05745)	1287040..1287333	-	294	WP_009580422.1	putative addiction module antidote protein	Antitoxin
KM433_RS05770 (KM433_05750)	1287336..1287632	-	297	WP_009580424.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KM433_RS05775 (KM433_05755)	1287797..1289185	-	1389	WP_009580426.1	sigma-54 dependent transcriptional regulator	-
KM433_RS05780 (KM433_05760)	1289260..1290873	-	1614	WP_009580428.1	HAMP domain-containing sensor histidine kinase	-
KM433_RS05785 (KM433_05765)	1291172..1292335	+	1164	WP_009580518.1	ADP-forming succinate--CoA ligase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1284111..1297848	13737

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (39-91)

Antitoxin

• Uncharacterized protein HI1420 (5-92)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11153.73 Da
• Isoelectric Point: 10.3355

>T205013 WP_009580424.1 NZ_CP076257:c1287632-1287336 [Xanthomonas translucens pv. graminis]
VIELKQTETFRKWRTKLKDERARTAIALRLTRLAYGHAGDTEAVGQGISELRIHHGPGYRVYFQRRGDTIIVLLCGGDKS
SQAKDIKTAKRLADEWSE

Nucleotide

• Length: 297 bp

>T205013 NZ_CP101366:6864-7118 [Salmonella enterica]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10376.69 Da
• Isoelectric Point: 4.9890

>AT205013 WP_009580422.1 NZ_CP076257:c1287333-1287040 [Xanthomonas translucens pv. graminis]
MTENLTTYDPAEDLNSDQAIADFMAGAFATNDTGFIAHALGIVARAKGMTQIARQTGLSREQLYRSFSEDGNPTLRTTLA
VMKALGIELTAKAAVTH

Nucleotide

• Length: 294 bp

>AT205013 NZ_CP101366:7115-7357 [Salmonella enterica]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGAAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Structures

Toxin

• AlphaFold DB: A0A1M4L1P2