Detailed information of TA system TA205009

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 3449491..3450083
• Replicon: chromosome
• Accession: NZ_CP076254
• Organism: Xanthomonas translucens pv. graminis strain LMG 726

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A1M4L1P2
• Locus tag: KFS84_RS15375
• Protein ID: WP_009580424.1
• Coordinates: 3449491..3449787 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: KFS84_RS15380
• Protein ID: WP_009580422.1
• Coordinates: 3449790..3450083 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFS84_RS15360 (KFS84_15300)	3444788..3445951	-	1164	WP_009580518.1	ADP-forming succinate--CoA ligase subunit beta	-
KFS84_RS15365 (KFS84_15305)	3446250..3447863	+	1614	WP_009580428.1	HAMP domain-containing sensor histidine kinase	-
KFS84_RS15370 (KFS84_15310)	3447938..3449326	+	1389	WP_009580426.1	sigma-54 dependent transcriptional regulator	-
KFS84_RS15375 (KFS84_15315)	3449491..3449787	+	297	WP_009580424.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KFS84_RS15380 (KFS84_15320)	3449790..3450083	+	294	WP_009580422.1	putative addiction module antidote protein	Antitoxin
KFS84_RS15385 (KFS84_15325)	3450111..3451847	-	1737	WP_009580420.1	type IV-A pilus assembly ATPase PilB	-
KFS84_RS15390 (KFS84_15330)	3452054..3452344	+	291	WP_009580418.1	nucleotidyltransferase family protein	-
KFS84_RS15395 (KFS84_15335)	3452341..3452661	+	321	WP_009580416.1	DUF86 domain-containing protein	-
KFS84_RS15400	3452761..3453012	+	252	WP_009580414.1	transposase	-
KFS84_RS15405	3453013..3453318	+	306	WP_231103532.1	hypothetical protein	-
KFS84_RS15410 (KFS84_15345)	3453716..3453961	-	246	WP_237654532.1	hypothetical protein	-
KFS84_RS15415 (KFS84_15350)	3454022..3454978	+	957	WP_009597007.1	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3449491..3470766	21275
	-	flank	IS/Tn	-	-	3454022..3454978	956

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (39-91)

Antitoxin

• Uncharacterized protein HI1420 (5-92)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11153.73 Da
• Isoelectric Point: 10.3355

>T205009 WP_009580424.1 NZ_CP076254:3449491-3449787 [Xanthomonas translucens pv. graminis]
VIELKQTETFRKWRTKLKDERARTAIALRLTRLAYGHAGDTEAVGQGISELRIHHGPGYRVYFQRRGDTIIVLLCGGDKS
SQAKDIKTAKRLADEWSE

Nucleotide

• Length: 297 bp

>T205009 NZ_CP101365:c4206890-4206606 [Salmonella enterica]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGCTTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGTAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10376.69 Da
• Isoelectric Point: 4.9890

>AT205009 WP_009580422.1 NZ_CP076254:3449790-3450083 [Xanthomonas translucens pv. graminis]
MTENLTTYDPAEDLNSDQAIADFMAGAFATNDTGFIAHALGIVARAKGMTQIARQTGLSREQLYRSFSEDGNPTLRTTLA
VMKALGIELTAKAAVTH

Nucleotide

• Length: 294 bp

>AT205009 NZ_CP101365:c4207122-4206880 [Salmonella enterica]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: A0A1M4L1P2