Detailed information of TA system TA205006

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 1378203..1378795
• Replicon: chromosome
• Accession: NZ_CP076253
• Organism: Xanthomonas translucens pv. graminis strain ART-Xtg2

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A1M4L1P2
• Locus tag: KM563_RS06175
• Protein ID: WP_009580424.1
• Coordinates: 1378499..1378795 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: KM563_RS06170
• Protein ID: WP_009580422.1
• Coordinates: 1378203..1378496 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KM563_RS06140 (KM563_06105)	1373896..1374306	+	411	WP_039955568.1	pilin	-
KM563_RS06145	1374968..1375273	-	306	WP_231103532.1	hypothetical protein	-
KM563_RS06150	1375274..1375525	-	252	WP_009580414.1	transposase	-
KM563_RS06155 (KM563_06115)	1375625..1375945	-	321	WP_009580416.1	DUF86 domain-containing protein	-
KM563_RS06160 (KM563_06120)	1375942..1376232	-	291	WP_009580418.1	nucleotidyltransferase family protein	-
KM563_RS06165 (KM563_06125)	1376439..1378175	+	1737	WP_009580420.1	type IV-A pilus assembly ATPase PilB	-
KM563_RS06170 (KM563_06130)	1378203..1378496	-	294	WP_009580422.1	putative addiction module antidote protein	Antitoxin
KM563_RS06175 (KM563_06135)	1378499..1378795	-	297	WP_009580424.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KM563_RS06180 (KM563_06140)	1378960..1380348	-	1389	WP_009580426.1	sigma-54 dependent transcriptional regulator	-
KM563_RS06185 (KM563_06145)	1380423..1382036	-	1614	WP_009580428.1	HAMP domain-containing sensor histidine kinase	-
KM563_RS06190 (KM563_06150)	1382335..1383498	+	1164	WP_009580518.1	ADP-forming succinate--CoA ligase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1375274..1389011	13737

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (39-91)

Antitoxin

• Uncharacterized protein HI1420 (5-92)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11153.73 Da
• Isoelectric Point: 10.3355

>T205006 WP_009580424.1 NZ_CP076253:c1378795-1378499 [Xanthomonas translucens pv. graminis]
VIELKQTETFRKWRTKLKDERARTAIALRLTRLAYGHAGDTEAVGQGISELRIHHGPGYRVYFQRRGDTIIVLLCGGDKS
SQAKDIKTAKRLADEWSE

Nucleotide

• Length: 297 bp

>T205006 NZ_CP101365:3491687-3491905 [Salmonella enterica]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACAATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10376.69 Da
• Isoelectric Point: 4.9890

>AT205006 WP_009580422.1 NZ_CP076253:c1378496-1378203 [Xanthomonas translucens pv. graminis]
MTENLTTYDPAEDLNSDQAIADFMAGAFATNDTGFIAHALGIVARAKGMTQIARQTGLSREQLYRSFSEDGNPTLRTTLA
VMKALGIELTAKAAVTH

Nucleotide

• Length: 294 bp

>AT205006 NZ_CP101365:3491285-3491659 [Salmonella enterica]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAAC
ATATTGCAACTTTCGCTCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A1M4L1P2