Detailed information of TA system TA205002

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 3337138..3337730
• Replicon: chromosome
• Accession: NZ_CP076251
• Organism: Xanthomonas translucens pv. phlei strain LMG 730

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A0K2ZF26
• Locus tag: KM547_RS14550
• Protein ID: WP_053833736.1
• Coordinates: 3337138..3337434 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: KM547_RS14555
• Protein ID: WP_009580422.1
• Coordinates: 3337437..3337730 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KM547_RS14535 (KM547_14475)	3332490..3333653	-	1164	WP_053833739.1	ADP-forming succinate--CoA ligase subunit beta	-
KM547_RS14540 (KM547_14480)	3333897..3335510	+	1614	WP_053838003.1	ATP-binding protein	-
KM547_RS14545 (KM547_14485)	3335585..3336973	+	1389	WP_003477091.1	sigma-54 dependent transcriptional regulator	-
KM547_RS14550 (KM547_14490)	3337138..3337434	+	297	WP_053833736.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KM547_RS14555 (KM547_14495)	3337437..3337730	+	294	WP_009580422.1	putative addiction module antidote protein	Antitoxin
KM547_RS14560 (KM547_14500)	3337758..3339494	-	1737	WP_053838004.1	type IV-A pilus assembly ATPase PilB	-
KM547_RS14565 (KM547_14505)	3339701..3339991	+	291	WP_009580418.1	nucleotidyltransferase family protein	-
KM547_RS14570 (KM547_14510)	3339988..3340359	+	372	WP_053838005.1	DUF86 domain-containing protein	-
KM547_RS14575 (KM547_14515)	3340495..3341052	+	558	WP_053838006.1	transposase	-
KM547_RS14580 (KM547_14520)	3341286..3341735	-	450	WP_053838007.1	pilin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (39-91)

Antitoxin

• Uncharacterized protein HI1420 (5-92)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11167.76 Da
• Isoelectric Point: 10.3355

>T205002 WP_053833736.1 NZ_CP076251:3337138-3337434 [Xanthomonas translucens pv. phlei]
VIELKQTETFRKWRTKLKDERARTAIALRLTRLAYGHAGDTEAVGQGISELRIHHGPGYRVYFQRRGDTIIVLLCGGDKS
TQAKDIKTAKRLADEWSE

Nucleotide

• Length: 297 bp

>T205002 NZ_CP101365:c1002567-1002040 [Salmonella enterica]
ATGATGTTTACAGAATGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGCGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATATGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTGTGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10376.69 Da
• Isoelectric Point: 4.9890

>AT205002 WP_009580422.1 NZ_CP076251:3337437-3337730 [Xanthomonas translucens pv. phlei]
MTENLTTYDPAEDLNSDQAIADFMAGAFATNDTGFIAHALGIVARAKGMTQIARQTGLSREQLYRSFSEDGNPTLRTTLA
VMKALGIELTAKAAVTH

Nucleotide

• Length: 294 bp

>AT205002 NZ_CP101365:c1002854-1002564 [Salmonella enterica]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTGCGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGCGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Structures

Toxin

• AlphaFold DB: A0A0K2ZF26