Detailed information of TA system TA204999

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 685168..685919
• Replicon: chromosome
• Accession: NZ_CP076251
• Organism: Xanthomonas translucens pv. phlei strain LMG 730

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: KM547_RS02970
• Protein ID: WP_080998904.1
• Coordinates: 685443..685919 (+)
• Length: 159 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A0K2ZF15
• Locus tag: KM547_RS02965
• Protein ID: WP_053837064.1
• Coordinates: 685168..685440 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KM547_RS02945 (KM547_02925)	680941..682209	+	1269	WP_053837060.1	MFS transporter	-
KM547_RS02950 (KM547_02930)	682238..683224	-	987	WP_053837061.1	NAD(P)-dependent oxidoreductase	-
KM547_RS02955 (KM547_02935)	683318..683890	+	573	WP_144422966.1	ANTAR domain-containing protein	-
KM547_RS02960 (KM547_02940)	683898..685007	+	1110	WP_053837063.1	CmpA/NrtA family ABC transporter substrate-binding protein	-
KM547_RS02965 (KM547_02945)	685168..685440	+	273	WP_053837064.1	DUF1778 domain-containing protein	Antitoxin
KM547_RS02970 (KM547_02950)	685443..685919	+	477	WP_080998904.1	GNAT family N-acetyltransferase	Toxin
KM547_RS02975 (KM547_02955)	686534..687703	+	1170	WP_186008100.1	MFS transporter	-
KM547_RS02980 (KM547_02960)	687765..690182	+	2418	WP_237651321.1	nitrite reductase large subunit NirB	-
KM547_RS02985 (KM547_02965)	690186..690551	+	366	WP_003469752.1	nitrite reductase small subunit NirD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-89)

Sequences

Toxin

Protein

• Length: 159 a.a.
• Molecular weight: 17303.01 Da
• Isoelectric Point: 8.0807

>T204999 WP_080998904.1 NZ_CP076251:685443-685919 [Xanthomonas translucens pv. phlei]
MSLTVPGPLNDVHRLDDFQCSAPELTRWLLERAHQNQLSGASRCFVVCDGQQRVVGYYALAAGVVAHEDAPGRIHRNMPN
PVPVIVLGRLAVHVDWAGQGVGRGLLKDAVQRTLQVCEQVGARALLCHAIDEAAKAFYLKHGFIASPIHDRTVMLPLR

Nucleotide

• Length: 477 bp

>T204999 NZ_CP101365:200101-200586 [Salmonella enterica]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9957.43 Da
• Isoelectric Point: 7.1666

>AT204999 WP_053837064.1 NZ_CP076251:685168-685440 [Xanthomonas translucens pv. phlei]
MSAVTSKVINFRAPAAKQALIDHAVEVSGMNRTEFILDAVCEKAREVLADQTRFALSPQQLQRFNALLDAPLESNAAIRR
LLSTPAPWEH

Nucleotide

• Length: 273 bp

>AT204999 NZ_CP101365:199826-200113 [Salmonella enterica]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCAGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0K2ZF15