Detailed information of TA system TA204998

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 3584171..3584763
• Replicon: chromosome
• Accession: NZ_CP076250
• Organism: Xanthomonas translucens pv. poae strain LMG 728

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A0K2ZF26
• Locus tag: KM539_RS15550
• Protein ID: WP_053833736.1
• Coordinates: 3584171..3584467 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: KM539_RS15555
• Protein ID: WP_237653908.1
• Coordinates: 3584464..3584763 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KM539_RS15535 (KM539_15500)	3579519..3580682	-	1164	WP_053833739.1	ADP-forming succinate--CoA ligase subunit beta	-
KM539_RS15540 (KM539_15505)	3580924..3582543	+	1620	WP_053839882.1	ATP-binding protein	-
KM539_RS15545 (KM539_15510)	3582618..3584006	+	1389	WP_053839881.1	sigma-54 dependent transcriptional regulator	-
KM539_RS15550 (KM539_15515)	3584171..3584467	+	297	WP_053833736.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KM539_RS15555 (KM539_15520)	3584464..3584763	+	300	WP_237653908.1	putative addiction module antidote protein	Antitoxin
KM539_RS15560 (KM539_15525)	3584791..3586527	-	1737	WP_053839880.1	type IV-A pilus assembly ATPase PilB	-
KM539_RS15565 (KM539_15530)	3586715..3587020	+	306	WP_237653910.1	hypothetical protein	-
KM539_RS15570 (KM539_15535)	3587254..3587712	-	459	WP_053839879.1	pilin	-
KM539_RS15575 (KM539_15540)	3588094..3589353	+	1260	WP_053839878.1	type II secretion system F family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (39-91)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-92)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11167.76 Da
• Isoelectric Point: 10.3355

>T204998 WP_053833736.1 NZ_CP076250:3584171-3584467 [Xanthomonas translucens pv. poae]
VIELKQTETFRKWRTKLKDERARTAIALRLTRLAYGHAGDTEAVGQGISELRIHHGPGYRVYFQRRGDTIIVLLCGGDKS
TQAKDIKTAKRLADEWSE

Nucleotide

• Length: 297 bp

>T204998 NZ_CP101311:82782-83198 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGCATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10608.99 Da
• Isoelectric Point: 4.9890

>AT204998 WP_237653908.1 NZ_CP076250:3584464-3584763 [Xanthomonas translucens pv. poae]
MTMTENLTTYDPAEDLNSDQAIADFMAGAFATNDTGFIAHALGIVARAKGMTQIARQTGLSREQLYRSFSEDGNPTLRTT
LAVMKALGIELTAKAAVTH

Nucleotide

• Length: 300 bp

>AT204998 NZ_CP101311:82555-82785 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0K2ZF26