Detailed information of TA system TA204985

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 53752..54277
• Replicon: plasmid pO157
• Accession: NZ_CP076246
• Organism: Escherichia coli O157:H7 strain Balcarce_24.2

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: V0SSI5
• Locus tag: JKN64_RS27460
• Protein ID: WP_001159868.1
• Coordinates: 53972..54277 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1PPD8
• Locus tag: JKN64_RS27455
• Protein ID: WP_000813634.1
• Coordinates: 53752..53970 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKN64_RS27425 (49035)	49035..49265	+	231	WP_032171660.1	hypothetical protein	-
JKN64_RS27430 (49386)	49386..50126	+	741	WP_001066920.1	tyrosine-type recombinase/integrase	-
JKN64_RS27435 (50411)	50411..51388	-	978	WP_000361615.1	RepB family plasmid replication initiator protein	-
JKN64_RS27440 (51796)	51796..51996	-	201	WP_000708307.1	hypothetical protein	-
JKN64_RS27445 (51993)	51993..52613	-	621	WP_001248529.1	hypothetical protein	-
JKN64_RS27450 (52610)	52610..53293	-	684	WP_010891291.1	DNA-binding protein	-
JKN64_RS27455 (53752)	53752..53970	+	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
JKN64_RS27460 (53972)	53972..54277	+	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	Toxin
JKN64_RS27465 (54278)	54278..55084	+	807	WP_000016989.1	site-specific integrase	-
JKN64_RS27475 (56982)	56982..57320	+	339	WP_071525396.1	RepB family plasmid replication initiator protein	-
JKN64_RS27480 (57908)	57908..59074	+	1167	WP_000772446.1	plasmid-partitioning protein SopA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB	1..95209	95209

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11706.51 Da
• Isoelectric Point: 6.4674

>T204985 WP_001159868.1 NZ_CP076246:53972-54277 [Escherichia coli O157:H7]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T204985 NZ_CP101310:1107946-1108257 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.34 Da
• Isoelectric Point: 4.7405

>AT204985 WP_000813634.1 NZ_CP076246:53752-53970 [Escherichia coli O157:H7]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT204985 NZ_CP101310:1108254-1108673 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACAATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• PDB: 4VUB
• PDB: 2VUB
• PDB: 7EPI
• PDB: 7EPG
• PDB: 3VUB
• PDB: 1X75
• PDB: 1VUB
• PDB: 7EPJ
• PDB: 3HPW
• PDB: 3G7Z

Antitoxin

• PDB: 3TCJ
• PDB: 2H3A
• PDB: 2ADN
• PDB: 2ADL
• PDB: 3HPW
• PDB: 2H3C
• PDB: 3G7Z
• AlphaFold DB: A0A829CQY2