Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 1782..2341 Replicon plasmid pO157
Accession NZ_CP076246
Organism Escherichia coli O157:H7 strain Balcarce_24.2

Toxin (Protein)


Gene name relE Uniprot ID O82928
Locus tag JKN64_RS27200 Protein ID WP_000421248.1
Coordinates 2066..2341 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag JKN64_RS27195 Protein ID WP_001178089.1
Coordinates 1782..2066 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKN64_RS27190 (13) 13..870 + 858 WP_000130945.1 incFII family plasmid replication initiator RepA -
JKN64_RS27195 (1782) 1782..2066 + 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
JKN64_RS27200 (2066) 2066..2341 + 276 WP_000421248.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JKN64_RS27205 (2436) 2436..2642 + 207 WP_001105064.1 hypothetical protein -
JKN64_RS27210 (2742) 2742..2957 - 216 Protein_5 transposase -
JKN64_RS27215 (3001) 3001..3567 - 567 Protein_6 IS3-like element IS600 family transposase -
JKN64_RS27220 (3567) 3567..3983 - 417 Protein_7 IS91 family transposase -
JKN64_RS27225 (3983) 3983..4168 - 186 WP_001302179.1 hypothetical protein -
JKN64_RS27230 (4345) 4345..6555 + 2211 WP_000592771.1 catalase/peroxidase KatP -
JKN64_RS27235 (6599) 6599..6988 + 390 WP_001172748.1 cytochrome b562 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB 1..95209 95209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10735.42 Da        Isoelectric Point: 9.9324

>T204984 WP_000421248.1 NZ_CP076246:2066-2341 [Escherichia coli O157:H7]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T204984 NZ_CP101310:1039671-1040006 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTATGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT204984 WP_001178089.1 NZ_CP076246:1782-2066 [Escherichia coli O157:H7]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT204984 NZ_CP101310:1039423-1039671 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z6FAZ0

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References