Detailed information of TA system TA204979

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4611353..4611903
• Replicon: chromosome
• Accession: NZ_CP076245
• Organism: Escherichia coli O157:H7 strain Balcarce_24.2

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: JKN64_RS23180
• Protein ID: WP_001160975.1
• Coordinates: 4611353..4611667 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: JKN64_RS23185
• Protein ID: WP_000125566.1
• Coordinates: 4611670..4611903 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKN64_RS23155 (4607028)	4607028..4608314	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
JKN64_RS23160 (4608314)	4608314..4609303	+	990	WP_000241259.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
JKN64_RS23165 (4609300)	4609300..4610121	+	822	WP_001065380.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
JKN64_RS23170 (4610124)	4610124..4610501	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
JKN64_RS23175 (4610508)	4610508..4611356	+	849	WP_000257187.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
JKN64_RS23180 (4611353)	4611353..4611667	-	315	WP_001160975.1	CcdB family protein	Toxin
JKN64_RS23185 (4611670)	4611670..4611903	-	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
JKN64_RS23190 (4611989)	4611989..4612468	-	480	WP_000624372.1	type 3 dihydrofolate reductase	-
JKN64_RS23195 (4612660)	4612660..4614522	-	1863	WP_000377184.1	glutathione-regulated potassium-efflux system protein KefC	-
JKN64_RS23200 (4614515)	4614515..4615045	-	531	WP_000600700.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
JKN64_RS23205 (4615153)	4615153..4616484	-	1332	WP_001183177.1	MFS transporter	-
JKN64_RS23210 (4616542)	4616542..4616829	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T204979 WP_001160975.1 NZ_CP076245:c4611667-4611353 [Escherichia coli O157:H7]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T204979 NZ_CP101310:346926-347453 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTCACTCTTGGGCGTCTGGCAATTGATCATTCAATACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCAGTAGGTATTCACGGTCTTTTTGTAG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCGTTGGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT204979 WP_000125566.1 NZ_CP076245:c4611903-4611670 [Escherichia coli O157:H7]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT204979 NZ_CP101310:346653-346919 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGTAAAACGTCTTCAGGGAATGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9