Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RelE-HTH_XRE
Location 3657503..3658208 Replicon chromosome
Accession NZ_CP076245
Organism Escherichia coli O157:H7 strain Balcarce_24.2

Toxin (Protein)


Gene name relE Uniprot ID Q8X3N0
Locus tag JKN64_RS18740 Protein ID WP_000539519.1
Coordinates 3657503..3657889 (+) Length 129 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID -
Locus tag JKN64_RS18745 Protein ID WP_001743136.1
Coordinates 3657879..3658208 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKN64_RS18720 (3653507) 3653507..3654133 + 627 WP_001295292.1 glutathione S-transferase GstB -
JKN64_RS18725 (3654130) 3654130..3655245 - 1116 WP_000554959.1 aldose sugar dehydrogenase YliI -
JKN64_RS18730 (3655356) 3655356..3655739 - 384 WP_000497137.1 biofilm formation regulator BssR -
JKN64_RS18735 (3655952) 3655952..3657277 + 1326 WP_000049367.1 30S ribosomal protein S12 methylthiotransferase RimO -
JKN64_RS18740 (3657503) 3657503..3657889 + 387 WP_000539519.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JKN64_RS18745 (3657879) 3657879..3658208 + 330 WP_001743136.1 DNA-binding transcriptional regulator Antitoxin
JKN64_RS18750 (3658278) 3658278..3659606 - 1329 WP_000086905.1 GGDEF domain-containing protein -
JKN64_RS18755 (3659614) 3659614..3661962 - 2349 WP_000950320.1 EAL domain-containing protein -
JKN64_RS18760 (3662140) 3662140..3663051 - 912 WP_001236044.1 glutathione ABC transporter permease GsiD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(1-124)

Antitoxin

Cro/C1-type helix-turn-helix domain

(53-94)


Sequences

Toxin        


Download         Length: 129 a.a.        Molecular weight: 14279.42 Da        Isoelectric Point: 9.9296

>T204976 WP_000539519.1 NZ_CP076245:3657503-3657889 [Escherichia coli O157:H7]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGSKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Download         Length: 387 bp

>T204976 NZ_CP101305:c5190460-5190149 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11996.10 Da        Isoelectric Point: 10.0101

>AT204976 WP_001743136.1 NZ_CP076245:3657879-3658208 [Escherichia coli O157:H7]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESISK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Download         Length: 330 bp

>AT204976 NZ_CP101305:c5190148-5189858 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References