Detailed information of TA system TA204973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2702485..2703123
• Replicon: chromosome
• Accession: NZ_CP076245
• Organism: Escherichia coli O157:H7 strain Balcarce_24.2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: JKN64_RS13565
• Protein ID: WP_000813794.1
• Coordinates: 2702947..2703123 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JKN64_RS13560
• Protein ID: WP_001270286.1
• Coordinates: 2702485..2702901 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKN64_RS13540 (2697637)	2697637..2698578	-	942	WP_001251315.1	ABC transporter permease	-
JKN64_RS13545 (2698579)	2698579..2699592	-	1014	WP_000220402.1	ABC transporter ATP-binding protein	-
JKN64_RS13550 (2699610)	2699610..2700755	-	1146	WP_000047432.1	ABC transporter substrate-binding protein	-
JKN64_RS13555 (2701000)	2701000..2702406	-	1407	WP_000760654.1	PLP-dependent aminotransferase family protein	-
JKN64_RS13560 (2702485)	2702485..2702901	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
JKN64_RS13565 (2702947)	2702947..2703123	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
JKN64_RS13570 (2703345)	2703345..2703575	+	231	WP_000494244.1	YncJ family protein	-
JKN64_RS13575 (2703667)	2703667..2705628	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
JKN64_RS13580 (2705701)	2705701..2706237	-	537	WP_000429145.1	DNA-binding transcriptional regulator SutR	-
JKN64_RS13585 (2706290)	2706290..2707501	+	1212	WP_071525382.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T204973 WP_000813794.1 NZ_CP076245:c2703123-2702947 [Escherichia coli O157:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T204973 NZ_CP101305:4235092-4235370 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT204973 WP_001270286.1 NZ_CP076245:c2702901-2702485 [Escherichia coli O157:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT204973 NZ_CP101305:4234829-4235089 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATAATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• PDB: 6HPB