Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 365568..366199 Replicon chromosome
Accession NZ_CP076245
Organism Escherichia coli O157:H7 strain Balcarce_24.2

Toxin (Protein)


Gene name hicA Uniprot ID Q8X5S6
Locus tag JKN64_RS01695 Protein ID WP_001259386.1
Coordinates 365568..365843 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag JKN64_RS01700 Protein ID WP_000593555.1
Coordinates 365840..366199 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKN64_RS01680 (360572) 360572..361639 + 1068 WP_000361458.1 HlyD family secretion protein -
JKN64_RS01685 (361636) 361636..364371 + 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
JKN64_RS01690 (364371) 364371..365495 + 1125 WP_001301659.1 ABC transporter permease -
JKN64_RS01695 (365568) 365568..365843 + 276 WP_001259386.1 type II toxin-antitoxin system HicA family toxin Toxin
JKN64_RS01700 (365840) 365840..366199 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
JKN64_RS01705 (366249) 366249..367109 - 861 WP_001131788.1 class II aldolase -
JKN64_RS01710 (367141) 367141..367410 - 270 WP_000933060.1 HPr family phosphocarrier protein -
JKN64_RS01715 (367400) 367400..368908 - 1509 Protein_340 FGGY family carbohydrate kinase -
JKN64_RS01720 (368901) 368901..370259 - 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
JKN64_RS01725 (370336) 370336..370617 - 282 WP_000084025.1 PTS sugar transporter subunit IIB -
JKN64_RS01730 (370614) 370614..371087 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 356713..366199 9486


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10165.92 Da        Isoelectric Point: 10.7076

>T204957 WP_001259386.1 NZ_CP076245:365568-365843 [Escherichia coli O157:H7]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKLILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T204957 NZ_CP101305:c847086-846760 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCATCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACTCGCGA
CAATTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT204957 WP_000593555.1 NZ_CP076245:365840-366199 [Escherichia coli O157:H7]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT204957 NZ_CP101305:c847544-847176 [Escherichia coli]
GTGTCAGACACACTCCATGAGACAAATTATCCCAACGACAATCACGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I9WXY7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References