Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 608..1167 Replicon plasmid pO157
Accession NZ_CP076244
Organism Escherichia coli O157:H7 strain Balcarce_14.2

Toxin (Protein)


Gene name relE Uniprot ID O82928
Locus tag JKN69_RS27490 Protein ID WP_000421248.1
Coordinates 608..883 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag JKN69_RS27495 Protein ID WP_001178089.1
Coordinates 883..1167 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKN69_RS27485 (307) 307..513 - 207 WP_001105064.1 hypothetical protein -
JKN69_RS27490 (608) 608..883 - 276 WP_000421248.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JKN69_RS27495 (883) 883..1167 - 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
JKN69_RS27500 (2079) 2079..2936 - 858 WP_000130945.1 incFII family plasmid replication initiator RepA -
JKN69_RS27505 (2929) 2929..3003 - 75 WP_001370046.1 RepA leader peptide Tap -
JKN69_RS27510 (3239) 3239..3493 - 255 WP_000083831.1 replication regulatory protein RepA -
JKN69_RS27515 (3733) 3733..4071 - 339 WP_000766796.1 hypothetical protein -
JKN69_RS27520 (4109) 4109..4318 - 210 WP_001302200.1 hemolysin expression modulator Hha -
JKN69_RS27525 (4364) 4364..4825 - 462 WP_001233856.1 thermonuclease family protein -
JKN69_RS27530 (5070) 5070..5282 - 213 WP_001302189.1 ANR family transcriptional regulator -
JKN69_RS27535 (5415) 5415..5975 - 561 WP_000840472.1 fertility inhibition protein FinO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - toxB / hlyD / hlyB / hlyA / hlyC / exeG / exeE / stcE / espP 1..94595 94595


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10735.42 Da        Isoelectric Point: 9.9324

>T204956 WP_000421248.1 NZ_CP076244:c883-608 [Escherichia coli O157:H7]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T204956 NZ_CP101305:722381-722761 [Escherichia coli]
ATGAAAACATTACCCGACACTCACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCTCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGTCCCGTCGCAGTTAATCAACAGCATCGATATCCTCCGGGCCCGCAGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTAGGCAGGCATCCGGAGGAGGCGAAACAATGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT204956 WP_001178089.1 NZ_CP076244:c1167-883 [Escherichia coli O157:H7]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT204956 NZ_CP101305:721924-722292 [Escherichia coli]
GTGTCAGACACACTCCATGAGACAAATTATCCCAACGACAATCACGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGACGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGGCACTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGATATGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z6FAZ0

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References