Detailed information of TA system TA204951

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4629077..4629627
• Replicon: chromosome
• Accession: NZ_CP076243
• Organism: Escherichia coli O157:H7 strain Balcarce_14.2

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: JKN69_RS23425
• Protein ID: WP_001160975.1
• Coordinates: 4629077..4629391 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: JKN69_RS23430
• Protein ID: WP_000125566.1
• Coordinates: 4629394..4629627 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKN69_RS23400 (4624752)	4624752..4626038	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
JKN69_RS23405 (4626038)	4626038..4627027	+	990	WP_000241259.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
JKN69_RS23410 (4627024)	4627024..4627845	+	822	WP_001065380.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
JKN69_RS23415 (4627848)	4627848..4628225	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
JKN69_RS23420 (4628232)	4628232..4629080	+	849	WP_000257187.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
JKN69_RS23425 (4629077)	4629077..4629391	-	315	WP_001160975.1	CcdB family protein	Toxin
JKN69_RS23430 (4629394)	4629394..4629627	-	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
JKN69_RS23435 (4629713)	4629713..4630192	-	480	WP_000624372.1	type 3 dihydrofolate reductase	-
JKN69_RS23440 (4630384)	4630384..4632246	-	1863	WP_000377184.1	glutathione-regulated potassium-efflux system protein KefC	-
JKN69_RS23445 (4632239)	4632239..4632769	-	531	WP_000600700.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
JKN69_RS23450 (4632877)	4632877..4634208	-	1332	WP_001183177.1	MFS transporter	-
JKN69_RS23455 (4634266)	4634266..4634553	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T204951 WP_001160975.1 NZ_CP076243:c4629391-4629077 [Escherichia coli O157:H7]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T204951 NZ_CP101302:c5298421-5298110 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT204951 WP_000125566.1 NZ_CP076243:c4629627-4629394 [Escherichia coli O157:H7]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT204951 NZ_CP101302:c5298109-5297819 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9