Detailed information of TA system TA204945

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2788210..2788848
• Replicon: chromosome
• Accession: NZ_CP076243
• Organism: Escherichia coli O157:H7 strain Balcarce_14.2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: JKN69_RS14235
• Protein ID: WP_000813794.1
• Coordinates: 2788672..2788848 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JKN69_RS14230
• Protein ID: WP_001270286.1
• Coordinates: 2788210..2788626 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKN69_RS14210 (2783362)	2783362..2784303	-	942	WP_001251315.1	ABC transporter permease	-
JKN69_RS14215 (2784304)	2784304..2785317	-	1014	WP_000220402.1	ABC transporter ATP-binding protein	-
JKN69_RS14220 (2785335)	2785335..2786480	-	1146	WP_000047432.1	ABC transporter substrate-binding protein	-
JKN69_RS14225 (2786725)	2786725..2788131	-	1407	WP_000760654.1	PLP-dependent aminotransferase family protein	-
JKN69_RS14230 (2788210)	2788210..2788626	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
JKN69_RS14235 (2788672)	2788672..2788848	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
JKN69_RS14240 (2789070)	2789070..2789300	+	231	WP_000494244.1	YncJ family protein	-
JKN69_RS14245 (2789392)	2789392..2791353	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
JKN69_RS14250 (2791426)	2791426..2791962	-	537	WP_000429145.1	DNA-binding transcriptional regulator SutR	-
JKN69_RS14255 (2792054)	2792054..2793227	+	1174	Protein_2784	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T204945 WP_000813794.1 NZ_CP076243:c2788848-2788672 [Escherichia coli O157:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T204945 NZ_CP101302:c4298263-4297928 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTATTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT204945 WP_001270286.1 NZ_CP076243:c2788626-2788210 [Escherichia coli O157:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT204945 NZ_CP101302:c4298511-4298263 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• PDB: 6HPB