Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 938870..939524 Replicon chromosome
Accession NZ_CP076243
Organism Escherichia coli O157:H7 strain Balcarce_14.2

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag JKN69_RS04650 Protein ID WP_000244781.1
Coordinates 939117..939524 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JKN69_RS04645 Protein ID WP_000354046.1
Coordinates 938870..939136 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKN69_RS04625 (934958) 934958..936391 - 1434 WP_001310226.1 6-phospho-beta-glucosidase BglA -
JKN69_RS04630 (936436) 936436..936747 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
JKN69_RS04635 (936911) 936911..937570 + 660 WP_000250274.1 hemolysin III family protein -
JKN69_RS04640 (937647) 937647..938627 - 981 WP_000886053.1 tRNA-modifying protein YgfZ -
JKN69_RS04645 (938870) 938870..939136 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JKN69_RS04650 (939117) 939117..939524 + 408 WP_000244781.1 protein YgfX Toxin
JKN69_RS04655 (939564) 939564..940085 - 522 WP_001055874.1 flavodoxin FldB -
JKN69_RS04660 (940197) 940197..941093 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JKN69_RS04665 (941118) 941118..941828 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JKN69_RS04670 (941834) 941834..943567 + 1734 WP_000813185.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T204934 WP_000244781.1 NZ_CP076243:939117-939524 [Escherichia coli O157:H7]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T204934 NZ_CP101302:c1347418-1347200 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT204934 WP_000354046.1 NZ_CP076243:938870-939136 [Escherichia coli O157:H7]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT204934 NZ_CP101302:c1347818-1347444 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References