Detailed information of TA system TA204931

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 450752..451506
• Replicon: chromosome
• Accession: NZ_CP076243
• Organism: Escherichia coli O157:H7 strain Balcarce_14.2

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: JKN69_RS02135
• Protein ID: WP_001301452.1
• Coordinates: 450752..451237 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: Q8X700
• Locus tag: JKN69_RS02140
• Protein ID: WP_000801912.1
• Coordinates: 451228..451506 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKN69_RS02115 (445918)	445918..446676	+	759	WP_001302007.1	DeoR/GlpR family transcriptional regulator	-
JKN69_RS02120 (446658)	446658..448256	-	1599	WP_001232889.1	DNA-binding transcriptional regulator RtcR	-
JKN69_RS02125 (448444)	448444..449670	+	1227	WP_001105473.1	RNA-splicing ligase RtcB	-
JKN69_RS02130 (449674)	449674..450702	+	1029	WP_000827117.1	RNA 3'-terminal phosphate cyclase	-
JKN69_RS02135 (450752)	450752..451237	-	486	WP_001301452.1	GNAT family N-acetyltransferase	Toxin
JKN69_RS02140 (451228)	451228..451506	-	279	WP_000801912.1	DUF1778 domain-containing protein	Antitoxin
JKN69_RS02145 (451573)	451573..454278	-	2706	WP_000906970.1	HTH-type transcriptional regulator MalT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-92)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17650.57 Da
• Isoelectric Point: 9.4066

>T204931 WP_001301452.1 NZ_CP076243:c451237-450752 [Escherichia coli O157:H7]
MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSGLSRVYVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAP
ESLPVVVLGRLAIDQAWAGKGLGVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
E

Nucleotide

• Length: 486 bp

>T204931 NZ_CP101302:700166-700537 [Escherichia coli]
ATGCAAACTATATCATCACACCCGACACGGGCGACTCAGCCCTGCCTGTCACCCGTTGAAATCTGGCAGCGGTTACTGAC
TTGTCTGTTGTCGCAGCACTATGGTCTGACGTTGAATGACACACCGTTCAGTAATGAAACCACCATACTGGAACACATTG
ATGCTGGAGTATCGCTGTGCGATGCGGTGAACTTTCTTGTTGAAAAATATGAACGGGTAAGAATTGACTGTAATGATTGC
TCTGTCATGGAGAAGTCACCGTTCATCACCAGTATCGATATTCTACGTGCCCGGAAAGCCTCCGGTCTGATGAAACGCAA
CAGTCACAAAACTGTTACCAGGGTAACGGCAGGTCGACTTCAGGAGCTATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10420.94 Da
• Isoelectric Point: 4.6336

>AT204931 WP_000801912.1 NZ_CP076243:c451506-451228 [Escherichia coli O157:H7]
MKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACREAQDILLDQRLFILDDEQYDAFLAALDAPITAERQAK
INALMNRKSPWE

Nucleotide

• Length: 279 bp

>AT204931 NZ_CP101302:699762-700127 [Escherichia coli]
ATGAACAATCACTCAGAATCCGGTACAAAGCCGGAGAATCCCGCCTGCCAGCAGTGGGGGCTTAAATGTGCTATTACCCC
CTGTTTTGGTGCCCGCCTGGTACAGGAGGGCAACCGATTGCATTTTCTCTCTGACCGCGCAGGATTTAGCGGGGCATTTG
CCGTCGATGTTGCAATGCGCCTTGACCAGGCATTTCCGTTAATGATGAAGCAACTTGAGCTGATGCTGACCAGTGGAGAG
CTTAACCCTCGCCACCCACACTGCGTCACGCTTTACCACAATGGACTCACCTGTGAAGCCGATACACTAGGCTCCTGTGG
TTATGTCTACATTGCTATCTATCCTGAGCAAACTGAGCCGCAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LHB9