Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 359391..360022 Replicon chromosome
Accession NZ_CP076243
Organism Escherichia coli O157:H7 strain Balcarce_14.2

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag JKN69_RS01675 Protein ID WP_001259385.1
Coordinates 359391..359666 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag JKN69_RS01680 Protein ID WP_000593555.1
Coordinates 359663..360022 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKN69_RS01660 (354395) 354395..355462 + 1068 WP_000361458.1 HlyD family secretion protein -
JKN69_RS01665 (355459) 355459..358194 + 2736 WP_000149110.1 ribosome-associated ATPase/putative transporter RbbA -
JKN69_RS01670 (358194) 358194..359318 + 1125 WP_001301659.1 ABC transporter permease -
JKN69_RS01675 (359391) 359391..359666 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
JKN69_RS01680 (359663) 359663..360022 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
JKN69_RS01685 (360072) 360072..360932 - 861 WP_001131788.1 class II aldolase -
JKN69_RS01690 (360964) 360964..361233 - 270 WP_000933060.1 HPr family phosphocarrier protein -
JKN69_RS01695 (361223) 361223..362731 - 1509 WP_001301773.1 FGGY-family carbohydrate kinase -
JKN69_RS01700 (362724) 362724..364082 - 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
JKN69_RS01705 (364159) 364159..364440 - 282 WP_000084025.1 PTS sugar transporter subunit IIB -
JKN69_RS01710 (364437) 364437..364910 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T204929 WP_001259385.1 NZ_CP076243:359391-359666 [Escherichia coli O157:H7]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T204929 NZ_CP101302:c489140-488763 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CCACTACAGAACGGTAAATGACATTACCCTGGGTAAACGCACGGAGGCAAAACGATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT204929 WP_000593555.1 NZ_CP076243:359663-360022 [Escherichia coli O157:H7]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT204929 NZ_CP101302:c489561-489187 [Escherichia coli]
GTGTCAGGCACACTCCATGAGACAAATTATCCCAACGACAACAACGACCGCCCCTGGTGGGGGCTCCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAATTACATTACCTTGCCGACCGCGCCGGTATCAGGGGGCAGT
TTAGCGACGCGGATGCGTATCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References