Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 1781..2340 Replicon plasmid pO157
Accession NZ_CP076242
Organism Escherichia coli O157:H7 strain Vac_07.1

Toxin (Protein)


Gene name relE Uniprot ID O82928
Locus tag JKN65_RS27080 Protein ID WP_000421248.1
Coordinates 2065..2340 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag JKN65_RS27075 Protein ID WP_001178089.1
Coordinates 1781..2065 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKN65_RS27070 (13) 13..870 + 858 WP_000130945.1 incFII family plasmid replication initiator RepA -
JKN65_RS27075 (1781) 1781..2065 + 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
JKN65_RS27080 (2065) 2065..2340 + 276 WP_000421248.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JKN65_RS27085 (2435) 2435..2641 + 207 WP_001105064.1 hypothetical protein -
JKN65_RS27090 (2741) 2741..2956 - 216 Protein_5 transposase -
JKN65_RS27095 (3000) 3000..3566 - 567 Protein_6 IS3-like element IS600 family transposase -
JKN65_RS27100 (3566) 3566..3982 - 417 Protein_7 IS91 family transposase -
JKN65_RS27105 (3982) 3982..4167 - 186 WP_001302179.1 hypothetical protein -
JKN65_RS27110 (4344) 4344..6554 + 2211 WP_000592771.1 catalase/peroxidase KatP -
JKN65_RS27115 (6598) 6598..6987 + 390 WP_001172748.1 cytochrome b562 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB 1..94989 94989


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10735.42 Da        Isoelectric Point: 9.9324

>T204927 WP_000421248.1 NZ_CP076242:2065-2340 [Escherichia coli O157:H7]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T204927 NZ_CP101295:c28965-28549 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGCATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATAAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT204927 WP_001178089.1 NZ_CP076242:1781-2065 [Escherichia coli O157:H7]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT204927 NZ_CP101295:c29192-28962 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z6FAZ0

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References