Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4162782..4163400 Replicon chromosome
Accession NZ_CP076241
Organism Escherichia coli O157:H7 strain Vac_07.1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JKN65_RS21205 Protein ID WP_001291435.1
Coordinates 4163182..4163400 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JKN65_RS21200 Protein ID WP_000344800.1
Coordinates 4162782..4163156 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKN65_RS21190 (4157871) 4157871..4159064 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JKN65_RS21195 (4159087) 4159087..4162236 + 3150 WP_001132452.1 efflux RND transporter permease AcrB -
JKN65_RS21200 (4162782) 4162782..4163156 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JKN65_RS21205 (4163182) 4163182..4163400 + 219 WP_001291435.1 HHA domain-containing protein Toxin
JKN65_RS21210 (4163572) 4163572..4164123 + 552 WP_000102553.1 maltose O-acetyltransferase -
JKN65_RS21215 (4164239) 4164239..4164709 + 471 WP_000136192.1 YlaC family protein -
JKN65_RS21220 (4164873) 4164873..4166423 + 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JKN65_RS21225 (4166465) 4166465..4166818 - 354 WP_000878140.1 DUF1428 domain-containing protein -
JKN65_RS21235 (4167197) 4167197..4167508 + 312 WP_000409911.1 MGMT family protein -
JKN65_RS21240 (4167541) 4167541..4168113 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T204920 WP_001291435.1 NZ_CP076241:4163182-4163400 [Escherichia coli O157:H7]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T204920 NZ_CP101292:c3660500-3660249 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT204920 WP_000344800.1 NZ_CP076241:4162782-4163156 [Escherichia coli O157:H7]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT204920 NZ_CP101292:c3660252-3659998 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References