Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RelE-HTH_XRE
Location 3708056..3708761 Replicon chromosome
Accession NZ_CP076241
Organism Escherichia coli O157:H7 strain Vac_07.1

Toxin (Protein)


Gene name relE Uniprot ID Q8X3N0
Locus tag JKN65_RS19100 Protein ID WP_000539519.1
Coordinates 3708056..3708442 (+) Length 129 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID -
Locus tag JKN65_RS19105 Protein ID WP_001280945.1
Coordinates 3708432..3708761 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKN65_RS19080 (3704060) 3704060..3704686 + 627 WP_001295292.1 glutathione S-transferase GstB -
JKN65_RS19085 (3704683) 3704683..3705798 - 1116 WP_000554959.1 aldose sugar dehydrogenase YliI -
JKN65_RS19090 (3705909) 3705909..3706292 - 384 WP_000497137.1 biofilm formation regulator BssR -
JKN65_RS19095 (3706505) 3706505..3707830 + 1326 WP_000049367.1 30S ribosomal protein S12 methylthiotransferase RimO -
JKN65_RS19100 (3708056) 3708056..3708442 + 387 WP_000539519.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JKN65_RS19105 (3708432) 3708432..3708761 + 330 WP_001280945.1 DNA-binding transcriptional regulator Antitoxin
JKN65_RS19110 (3708831) 3708831..3710159 - 1329 WP_000086905.1 GGDEF domain-containing protein -
JKN65_RS19115 (3710167) 3710167..3712515 - 2349 WP_000950320.1 EAL domain-containing protein -
JKN65_RS19120 (3712693) 3712693..3713604 - 912 WP_001236044.1 glutathione ABC transporter permease GsiD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(1-124)

Antitoxin

Cro/C1-type helix-turn-helix domain

(53-94)


Sequences

Toxin        


Download         Length: 129 a.a.        Molecular weight: 14279.42 Da        Isoelectric Point: 9.9296

>T204919 WP_000539519.1 NZ_CP076241:3708056-3708442 [Escherichia coli O157:H7]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGSKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Download         Length: 387 bp

>T204919 NZ_CP101292:c3660252-3659998 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11982.08 Da        Isoelectric Point: 10.0101

>AT204919 WP_001280945.1 NZ_CP076241:3708432-3708761 [Escherichia coli O157:H7]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Download         Length: 330 bp

>AT204919 NZ_CP101292:c3660500-3660249 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References