Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2794557..2795195 Replicon chromosome
Accession NZ_CP076241
Organism Escherichia coli O157:H7 strain Vac_07.1

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag JKN65_RS14260 Protein ID WP_000813794.1
Coordinates 2795019..2795195 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag JKN65_RS14255 Protein ID WP_001270286.1
Coordinates 2794557..2794973 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKN65_RS14235 (2789709) 2789709..2790650 - 942 WP_001251315.1 ABC transporter permease -
JKN65_RS14240 (2790651) 2790651..2791664 - 1014 WP_000220402.1 ABC transporter ATP-binding protein -
JKN65_RS14245 (2791682) 2791682..2792827 - 1146 WP_000047432.1 ABC transporter substrate-binding protein -
JKN65_RS14250 (2793072) 2793072..2794478 - 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
JKN65_RS14255 (2794557) 2794557..2794973 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
JKN65_RS14260 (2795019) 2795019..2795195 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
JKN65_RS14265 (2795417) 2795417..2795647 + 231 WP_000494244.1 YncJ family protein -
JKN65_RS14270 (2795739) 2795739..2797700 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
JKN65_RS14275 (2797773) 2797773..2798309 - 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
JKN65_RS14280 (2798401) 2798401..2799574 + 1174 Protein_2793 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T204916 WP_000813794.1 NZ_CP076241:c2795195-2795019 [Escherichia coli O157:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T204916 NZ_CP101292:3429001-3429279 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT204916 WP_001270286.1 NZ_CP076241:c2794973-2794557 [Escherichia coli O157:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT204916 NZ_CP101292:3428808-3428999 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References