Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2635627..2636098 Replicon chromosome
Accession NZ_CP076241
Organism Escherichia coli O157:H7 strain Vac_07.1

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag JKN65_RS13525 Protein ID WP_001303511.1
Coordinates 2635627..2635905 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag JKN65_RS13530 Protein ID WP_001302048.1
Coordinates 2635907..2636098 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKN65_RS13500 (2632563) 2632563..2633648 - 1086 Protein_2637 exonuclease -
JKN65_RS13505 (2633741) 2633741..2633932 - 192 WP_001090200.1 DUF1482 family protein -
JKN65_RS13510 (2633929) 2633929..2634117 - 189 WP_000449192.1 cell division inhibition protein DicB -
JKN65_RS13515 (2634686) 2634686..2634904 - 219 WP_001171930.1 protein YdfC -
JKN65_RS13520 (2634976) 2634976..2635275 - 300 WP_001240334.1 hypothetical protein -
JKN65_RS13525 (2635627) 2635627..2635905 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JKN65_RS13530 (2635907) 2635907..2636098 - 192 WP_001302048.1 hypothetical protein Antitoxin
JKN65_RS13535 (2636119) 2636119..2636490 - 372 WP_001169686.1 hypothetical protein -
JKN65_RS13540 (2636588) 2636588..2636659 + 72 Protein_2645 hypothetical protein -
JKN65_RS13545 (2636712) 2636712..2637925 + 1214 WP_162829202.1 IS3-like element IS1203 family transposase -
JKN65_RS13550 (2637981) 2637981..2638346 + 366 WP_000279796.1 HNH endonuclease -
JKN65_RS13555 (2638639) 2638639..2639202 + 564 WP_000958392.1 terminase small subunit -
JKN65_RS13560 (2639199) 2639199..2640860 + 1662 WP_001303179.1 terminase large subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2614306..2664924 50618
- inside IScluster/Tn - - 2631299..2637925 6626


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T204915 WP_001303511.1 NZ_CP076241:c2635905-2635627 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T204915 NZ_CP101292:c3109682-3109395 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT204915 WP_001302048.1 NZ_CP076241:c2636098-2635907 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT204915 NZ_CP101292:c3109921-3109682 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References