Detailed information of TA system TA204901

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 417772..418572
• Replicon: chromosome
• Accession: NZ_CP076241
• Organism: Escherichia coli O157:H7 strain Vac_07.1

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: A0A891SNC1
• Locus tag: JKN65_RS02030
• Protein ID: WP_000342448.1
• Coordinates: 418045..418572 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: F4NNI1
• Locus tag: JKN65_RS02025
• Protein ID: WP_001277108.1
• Coordinates: 417772..418038 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKN65_RS02005 (413430)	413430..414098	+	669	WP_000617723.1	cell division ATP-binding protein FtsE	-
JKN65_RS02010 (414091)	414091..415149	+	1059	WP_001042003.1	permease-like cell division protein FtsX	-
JKN65_RS02015 (415394)	415394..416248	+	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
JKN65_RS02020 (416519)	416519..417622	+	1104	WP_001021996.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
JKN65_RS02025 (417772)	417772..418038	+	267	WP_001277108.1	type II toxin-antitoxin system antitoxin AtaR	Antitoxin
JKN65_RS02030 (418045)	418045..418572	+	528	WP_000342448.1	type II toxin-antitoxin system toxin acetyltransferase AtaT	Toxin
JKN65_RS02035 (418569)	418569..418952	-	384	WP_000778769.1	aspartate 1-decarboxylase autocleavage activator PanM	-
JKN65_RS02040 (419376)	419376..420485	+	1110	WP_001301528.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
JKN65_RS02045 (420533)	420533..421459	+	927	WP_001295111.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
JKN65_RS02050 (421456)	421456..422733	+	1278	WP_000803797.1	branched chain amino acid ABC transporter permease LivM	-
JKN65_RS02055 (422730)	422730..423497	+	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19692.68 Da
• Isoelectric Point: 7.3232

>T204901 WP_000342448.1 NZ_CP076241:418045-418572 [Escherichia coli O157:H7]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T204901 NZ_CP101292:412725-412898 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9910.31 Da
• Isoelectric Point: 10.0863

>AT204901 WP_001277108.1 NZ_CP076241:417772-418038 [Escherichia coli O157:H7]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT204901 NZ_CP101292:412401-412697 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• PDB: 6GTS

Antitoxin

• PDB: 6GTS
• PDB: 6AJN
• PDB: 6GTQ
• PDB: 6GTO
• PDB: 6GTR
• PDB: 6AJM
• AlphaFold DB: A0A829CN24