Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 375341..375972 Replicon chromosome
Accession NZ_CP076241
Organism Escherichia coli O157:H7 strain Vac_07.1

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag JKN65_RS01780 Protein ID WP_001259385.1
Coordinates 375341..375616 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag JKN65_RS01785 Protein ID WP_000593555.1
Coordinates 375613..375972 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKN65_RS01765 (370345) 370345..371412 + 1068 WP_000361458.1 HlyD family secretion protein -
JKN65_RS01770 (371409) 371409..374144 + 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
JKN65_RS01775 (374144) 374144..375268 + 1125 WP_001301659.1 ABC transporter permease -
JKN65_RS01780 (375341) 375341..375616 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
JKN65_RS01785 (375613) 375613..375972 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
JKN65_RS01790 (376022) 376022..376882 - 861 WP_001131788.1 class II aldolase -
JKN65_RS01795 (376914) 376914..377183 - 270 WP_000933060.1 HPr family phosphocarrier protein -
JKN65_RS01800 (377173) 377173..378681 - 1509 WP_001301773.1 FGGY-family carbohydrate kinase -
JKN65_RS01805 (378674) 378674..380032 - 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
JKN65_RS01810 (380109) 380109..380390 - 282 WP_000084025.1 PTS sugar transporter subunit IIB -
JKN65_RS01815 (380387) 380387..380860 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 366485..375972 9487


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T204900 WP_001259385.1 NZ_CP076241:375341-375616 [Escherichia coli O157:H7]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T204900 NZ_CP101291:c2165631-2165305 [Alteromonas sp. LMIT006]
ATGAAAAGTGTATTTGTTGAATCAAGCATATTTGAAAAGTACCGAAATGAATATCTCAATGACGAAGAGTTTCGACTGTT
CCAAGCTGAACTAATGTCGAACCCGAAGCAAGGTGATGTAATTCAAGGTACAGGTGGTTTGCGAAAGATCCGAGTAGCAA
GTAAAGGTAAAGGAAAGCGTGGTGGTTCACGTGTTATCTATTATTTCCTCGATGAAAAGCGTCGTTTTTATTTGCTAACC
ATTTACGGCAAAAATGAAATGGCAGACTTAACCGCATATCAAAAGAAACAGTTAAAAGCTTTTATGGAGGCGTGGCGCAA
TGAGTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT204900 WP_000593555.1 NZ_CP076241:375613-375972 [Escherichia coli O157:H7]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT204900 NZ_CP101291:c2165312-2164998 [Alteromonas sp. LMIT006]
ATGAGTAATCGTGACCTATTTGCAGAGCTAAGTTCTGCACTTGTTGAAGCTAAGGAGCATTCAGAGGGTAAAGTGACTCT
GAAAACTCACCAAGTAAATGACATTAGTGAATTAGATATTTCGCCTGATGAAATAGTTAGTATTCGTGAGCAGTTTAATA
TGTCTCGTGGTGTATTTGCTCGTTTACTGCATACATCATCACGCACATTGGAAAACTGGGAGCAAGGGCGTAGCGCACCT
AATGGGCAAGCTGTTACTCTTCTAAAGTTGGTACAGCGTCATCCAGAAACACTGTCGCACATCGCAGAGCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References