Detailed information of TA system TA204898

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 1782..2341
• Replicon: plasmid pO157
• Accession: NZ_CP076239
• Organism: Escherichia coli O157:H7 strain 146N4

Toxin (Protein)

• Gene name: relE
• Uniprot ID: O82928
• Locus tag: JKN67_RS27670
• Protein ID: WP_000421248.1
• Coordinates: 2066..2341 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: JKN67_RS27665
• Protein ID: WP_001178089.1
• Coordinates: 1782..2066 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKN67_RS27660 (13)	13..870	+	858	WP_000130945.1	incFII family plasmid replication initiator RepA	-
JKN67_RS27665 (1782)	1782..2066	+	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
JKN67_RS27670 (2066)	2066..2341	+	276	WP_000421248.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JKN67_RS27675 (2436)	2436..2642	+	207	WP_001105064.1	hypothetical protein	-
JKN67_RS27680 (2742)	2742..2957	-	216	Protein_5	transposase	-
JKN67_RS27685 (3001)	3001..3567	-	567	Protein_6	IS3-like element IS600 family transposase	-
JKN67_RS27690 (3567)	3567..3983	-	417	Protein_7	IS91 family transposase	-
JKN67_RS27695 (3983)	3983..4168	-	186	WP_001302179.1	hypothetical protein	-
JKN67_RS27700 (4345)	4345..6555	+	2211	WP_000592771.1	catalase/peroxidase KatP	-
JKN67_RS27705 (6599)	6599..6988	+	390	WP_001172748.1	cytochrome b562	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	espP / espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB	1..95343	95343

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10735.42 Da
• Isoelectric Point: 9.9324

>T204898 WP_000421248.1 NZ_CP076239:2066-2341 [Escherichia coli O157:H7]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T204898 NZ_CP101290:c1328743-1327997 [Bacillus subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTATATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATCCGGAAAACTTGGTATTTGCTGTTTGTTCTAGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATACGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAGCCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT204898 WP_001178089.1 NZ_CP076239:1782-2066 [Escherichia coli O157:H7]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT204898 NZ_CP101290:c1327997-1327827 [Bacillus subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A2Z6FAZ0

Antitoxin

• AlphaFold DB: A0A731IHA8