Detailed information of TA system TA204896

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4971477..4972072
• Replicon: chromosome
• Accession: NZ_CP076237
• Organism: Escherichia coli O157:H7 strain 146N4

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: JKN67_RS24950
• Protein ID: WP_000239579.1
• Coordinates: 4971477..4971827 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: JKN67_RS24955
• Protein ID: WP_001223210.1
• Coordinates: 4971821..4972072 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKN67_RS24930 (4966923)	4966923..4967945	-	1023	WP_001301928.1	ABC transporter permease	-
JKN67_RS24935 (4967959)	4967959..4969461	-	1503	WP_000205806.1	sugar ABC transporter ATP-binding protein	-
JKN67_RS24940 (4969601)	4969601..4970557	-	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
JKN67_RS24945 (4970867)	4970867..4971397	+	531	WP_000055075.1	inorganic diphosphatase	-
JKN67_RS24950 (4971477)	4971477..4971827	-	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
JKN67_RS24955 (4971821)	4971821..4972072	-	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
JKN67_RS24960 (4972284)	4972284..4972625	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
JKN67_RS24965 (4972628)	4972628..4976407	-	3780	WP_000060930.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T204896 WP_000239579.1 NZ_CP076237:c4971827-4971477 [Escherichia coli O157:H7]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T204896 NZ_CP101283:2877632-2877964 [Phenylobacterium sp. LH3H17]
GTGCCGGACTACGTTCCGGACGCGGGGCACATAGTCTGGCTTGAGTTCGATCCCCAGGCGGGCCACGAGCAAGCCGGTCA
TCGCCCTGCCTTGGTCCTGTCGGCGGCGCGCTATAACCGACTTCGCGGGATGATGCTCTGCTGTCCCATGACGTCGCGGA
TCAAGGGGTACGTCTTCGAGGTGGTGGCGTCGGACGACCCGCCCAGCGTGATCCTTTCCGATCAGGTAAAAAGCCTGGAT
TGGCGAGCGAGGCGCGCCATTCGGAAAGGCTCGGTCGCGCCTGCGATCCTGGCGGAGGTCAAGGCCAAGATCGCCGCGCT
TCTACAACTTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT204896 WP_001223210.1 NZ_CP076237:c4972072-4971821 [Escherichia coli O157:H7]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT204896 NZ_CP101283:2877390-2877632 [Phenylobacterium sp. LH3H17]
GTGCGCGTCCAGATCAAGAAATGGGGCAACAGCGCCTCGGTGAGAATCCCGTCGTCGGTTATGGCCGCGGCGGACCTCAC
GGTTGACCAAGCCGTCGACGTTCGGGAGGAAGGCGGGCGCGTTGTTATCGAGCCTGTGCGCGCGCCGAAGTACGATCTCG
ATCAGATGCTGGATCGGATGACCCCCGACACCTTCCCGCAAGATGTCGACTTTGGACCACCCGTTGGACAGGAGGCCTGG
TAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8