Detailed information of TA system TA204893

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4677541..4678091
• Replicon: chromosome
• Accession: NZ_CP076237
• Organism: Escherichia coli O157:H7 strain 146N4

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: JKN67_RS23620
• Protein ID: WP_001160975.1
• Coordinates: 4677541..4677855 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: JKN67_RS23625
• Protein ID: WP_000125566.1
• Coordinates: 4677858..4678091 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKN67_RS23595 (4673216)	4673216..4674502	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
JKN67_RS23600 (4674502)	4674502..4675491	+	990	WP_000241259.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
JKN67_RS23605 (4675488)	4675488..4676309	+	822	WP_001065380.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
JKN67_RS23610 (4676312)	4676312..4676689	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
JKN67_RS23615 (4676696)	4676696..4677544	+	849	WP_065222301.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
JKN67_RS23620 (4677541)	4677541..4677855	-	315	WP_001160975.1	CcdB family protein	Toxin
JKN67_RS23625 (4677858)	4677858..4678091	-	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
JKN67_RS23630 (4678177)	4678177..4678656	-	480	WP_000624372.1	type 3 dihydrofolate reductase	-
JKN67_RS23635 (4678848)	4678848..4680710	-	1863	WP_000377184.1	glutathione-regulated potassium-efflux system protein KefC	-
JKN67_RS23640 (4680703)	4680703..4681233	-	531	WP_000600700.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
JKN67_RS23645 (4681341)	4681341..4682672	-	1332	WP_001183177.1	MFS transporter	-
JKN67_RS23650 (4682730)	4682730..4683017	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T204893 WP_001160975.1 NZ_CP076237:c4677855-4677541 [Escherichia coli O157:H7]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T204893 NZ_CP101275:137764-138180 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT204893 WP_000125566.1 NZ_CP076237:c4678091-4677858 [Escherichia coli O157:H7]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT204893 NZ_CP101275:137537-137767 [Escherichia coli]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9