Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4182220..4182838 Replicon chromosome
Accession NZ_CP076237
Organism Escherichia coli O157:H7 strain 146N4

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JKN67_RS21305 Protein ID WP_001291435.1
Coordinates 4182620..4182838 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JKN67_RS21300 Protein ID WP_000344800.1
Coordinates 4182220..4182594 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKN67_RS21290 (4177309) 4177309..4178502 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JKN67_RS21295 (4178525) 4178525..4181674 + 3150 WP_001132452.1 efflux RND transporter permease AcrB -
JKN67_RS21300 (4182220) 4182220..4182594 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JKN67_RS21305 (4182620) 4182620..4182838 + 219 WP_001291435.1 HHA domain-containing protein Toxin
JKN67_RS21310 (4183010) 4183010..4183561 + 552 WP_000102553.1 maltose O-acetyltransferase -
JKN67_RS21315 (4183677) 4183677..4184147 + 471 WP_000136192.1 YlaC family protein -
JKN67_RS21320 (4184311) 4184311..4185861 + 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JKN67_RS21325 (4185903) 4185903..4186256 - 354 WP_000878140.1 DUF1428 domain-containing protein -
JKN67_RS21335 (4186635) 4186635..4186946 + 312 WP_000409911.1 MGMT family protein -
JKN67_RS21340 (4186979) 4186979..4187551 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T204891 WP_001291435.1 NZ_CP076237:4182620-4182838 [Escherichia coli O157:H7]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T204891 NZ_CP101275:88944-89342 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATGAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT204891 WP_000344800.1 NZ_CP076237:4182220-4182594 [Escherichia coli O157:H7]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT204891 NZ_CP101275:88717-88944 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References