Detailed information of TA system TA204889

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 3561363..3561841
• Replicon: chromosome
• Accession: NZ_CP076237
• Organism: Escherichia coli O157:H7 strain 146N4

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: JKN67_RS18460
• Protein ID: WP_001303876.1
• Coordinates: 3561554..3561841 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: JKN67_RS18455
• Protein ID: WP_000536233.1
• Coordinates: 3561363..3561554 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKN67_RS18420 (3556767)	3556767..3557537	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
JKN67_RS18425 (3557559)	3557559..3558305	-	747	WP_000788751.1	ATP-binding protein	-
JKN67_RS18430 (3558312)	3558312..3559403	-	1092	WP_001205823.1	hypothetical protein	-
JKN67_RS18435 (3559482)	3559482..3559937	+	456	WP_000273724.1	hypothetical protein	-
JKN67_RS18440 (3560144)	3560144..3560569	-	426	WP_000693855.1	toxin YdaT family protein	-
JKN67_RS18445 (3560553)	3560553..3560825	-	273	WP_000887453.1	YdaS family helix-turn-helix protein	-
JKN67_RS18450 (3560934)	3560934..3561335	+	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
JKN67_RS18455 (3561363)	3561363..3561554	+	192	WP_000536233.1	hypothetical protein	Antitoxin
JKN67_RS18460 (3561554)	3561554..3561841	+	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JKN67_RS18465 (3562119)	3562119..3562274	+	156	WP_000379575.1	DUF1391 family protein	-
JKN67_RS28240 (3562276)	3562276..3562404	+	129	WP_000344963.1	protein YdfB	-
JKN67_RS18470 (3562416)	3562416..3562805	+	390	WP_000394511.1	hypothetical protein	-
JKN67_RS18475 (3562992)	3562992..3563177	-	186	WP_001133046.1	hypothetical protein	-
JKN67_RS18480 (3563751)	3563751..3563939	+	189	WP_000413705.1	cell division inhibition protein DicB	-
JKN67_RS18485 (3563936)	3563936..3564127	+	192	WP_001098307.1	DUF1482 family protein	-
JKN67_RS18490 (3564221)	3564221..3566692	+	2472	WP_000034474.1	exonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T204889 WP_001303876.1 NZ_CP076237:3561554-3561841 [Escherichia coli O157:H7]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T204889 NZ_CP101275:c76455-76168 [Escherichia coli]
ATGACTTATACAGTAAAATTCAGGGATGATGCGCTCAAAGAATGGCTGAAACTGGATAAATCCATTCAACAGCAGTTTGC
GAAAAAACTAAAGAAATGCAGCGAAAATCCTCATATTCCTTCCGCAAAACTGCGTGGATTAAAAGACTGCTACAAAATCA
AACTTCGCGCATCGGGGTTTCGTCTGGTCTATCAGGTTATTGATGACATGCTAATTATTGCGGTTGTCGCGGTAGGTAAA
CGTGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT204889 WP_000536233.1 NZ_CP076237:3561363-3561554 [Escherichia coli O157:H7]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT204889 NZ_CP101275:c76703-76452 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTATCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCGGAACTCTATGAAAGGATGC
TTGATGCGCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTGGAC
AGTTATTTATGA