Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2543820..2544291 Replicon chromosome
Accession NZ_CP076237
Organism Escherichia coli O157:H7 strain 146N4

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag JKN67_RS12770 Protein ID WP_001303511.1
Coordinates 2543820..2544098 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag JKN67_RS12775 Protein ID WP_001302048.1
Coordinates 2544100..2544291 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKN67_RS12740 (2539045) 2539045..2539296 - 252 WP_000005552.1 excisionase family protein -
JKN67_RS12745 (2539369) 2539369..2541840 - 2472 WP_000048458.1 exonuclease -
JKN67_RS12750 (2541933) 2541933..2542124 - 192 WP_001090200.1 DUF1482 family protein -
JKN67_RS12755 (2542121) 2542121..2542309 - 189 WP_000449192.1 cell division inhibition protein DicB -
JKN67_RS12760 (2542878) 2542878..2543096 - 219 WP_001171930.1 protein YdfC -
JKN67_RS12765 (2543168) 2543168..2543467 - 300 WP_001240334.1 hypothetical protein -
JKN67_RS12770 (2543820) 2543820..2544098 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JKN67_RS12775 (2544100) 2544100..2544291 - 192 WP_001302048.1 hypothetical protein Antitoxin
JKN67_RS12780 (2544312) 2544312..2544683 - 372 WP_001169686.1 hypothetical protein -
JKN67_RS12785 (2544781) 2544781..2545083 + 303 WP_000172738.1 transcriptional regulator -
JKN67_RS12790 (2545080) 2545080..2545505 + 426 WP_000693943.1 toxin YdaT family protein -
JKN67_RS12795 (2545528) 2545528..2546490 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
JKN67_RS12800 (2546497) 2546497..2547237 + 741 WP_000788938.1 ATP-binding protein -
JKN67_RS12805 (2547263) 2547263..2548032 + 770 Protein_2503 DUF1627 domain-containing protein -
JKN67_RS12810 (2548048) 2548048..2548443 + 396 WP_001118159.1 DUF977 family protein -
JKN67_RS12815 (2548500) 2548500..2549084 + 585 WP_000206793.1 DUF551 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T204878 WP_001303511.1 NZ_CP076237:c2544098-2543820 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T204878 NZ_CP101274:c2295080-2293758 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CAGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTAACGTTATTACCCGAAAA
TGAAACCATAACGCGCCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGGCTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTTAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTAAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT204878 WP_001302048.1 NZ_CP076237:c2544291-2544100 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT204878 NZ_CP101274:c2295346-2295080 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACACTATGCGACACGAATAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References