Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 456757..457511 Replicon chromosome
Accession NZ_CP076237
Organism Escherichia coli O157:H7 strain 146N4

Toxin (Protein)


Gene name ataT Uniprot ID -
Locus tag JKN67_RS02160 Protein ID WP_001301452.1
Coordinates 456757..457242 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID Q8X700
Locus tag JKN67_RS02165 Protein ID WP_000801912.1
Coordinates 457233..457511 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKN67_RS02140 (451923) 451923..452681 + 759 WP_001302007.1 DeoR/GlpR family transcriptional regulator -
JKN67_RS02145 (452663) 452663..454261 - 1599 WP_001232889.1 DNA-binding transcriptional regulator RtcR -
JKN67_RS02150 (454449) 454449..455675 + 1227 WP_001105473.1 RNA-splicing ligase RtcB -
JKN67_RS02155 (455679) 455679..456707 + 1029 WP_000827117.1 RNA 3'-terminal phosphate cyclase -
JKN67_RS02160 (456757) 456757..457242 - 486 WP_001301452.1 GNAT family N-acetyltransferase Toxin
JKN67_RS02165 (457233) 457233..457511 - 279 WP_000801912.1 DUF1778 domain-containing protein Antitoxin
JKN67_RS02170 (457578) 457578..460283 - 2706 WP_000906970.1 HTH-type transcriptional regulator MalT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-143)

Antitoxin

Vibrio phage ICP1, Orf50

(2-92)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17650.57 Da        Isoelectric Point: 9.4066

>T204865 WP_001301452.1 NZ_CP076237:c457242-456757 [Escherichia coli O157:H7]
MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSGLSRVYVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAP
ESLPVVVLGRLAIDQAWAGKGLGVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
E

Download         Length: 486 bp

>T204865 NZ_CP101270:c55324-55175 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCTACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10420.94 Da        Isoelectric Point: 4.6336

>AT204865 WP_000801912.1 NZ_CP076237:c457511-457233 [Escherichia coli O157:H7]
MKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACREAQDILLDQRLFILDDEQYDAFLAALDAPITAERQAK
INALMNRKSPWE

Download         Length: 279 bp

>AT204865 NZ_CP101270:55368-55429 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LHB9

References