Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 365396..366027 Replicon chromosome
Accession NZ_CP076237
Organism Escherichia coli O157:H7 strain 146N4

Toxin (Protein)


Gene name hicA Uniprot ID Q8X5S6
Locus tag JKN67_RS01700 Protein ID WP_001259386.1
Coordinates 365396..365671 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag JKN67_RS01705 Protein ID WP_000593555.1
Coordinates 365668..366027 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKN67_RS01685 (360400) 360400..361467 + 1068 WP_000361458.1 HlyD family secretion protein -
JKN67_RS01690 (361464) 361464..364199 + 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
JKN67_RS01695 (364199) 364199..365323 + 1125 WP_001301659.1 ABC transporter permease -
JKN67_RS01700 (365396) 365396..365671 + 276 WP_001259386.1 type II toxin-antitoxin system HicA family toxin Toxin
JKN67_RS01705 (365668) 365668..366027 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
JKN67_RS01710 (366077) 366077..366937 - 861 WP_001131788.1 class II aldolase -
JKN67_RS01715 (366969) 366969..367238 - 270 WP_000933060.1 HPr family phosphocarrier protein -
JKN67_RS01720 (367228) 367228..368736 - 1509 WP_001301773.1 FGGY-family carbohydrate kinase -
JKN67_RS01725 (368729) 368729..370087 - 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
JKN67_RS01730 (370164) 370164..370445 - 282 WP_000084025.1 PTS sugar transporter subunit IIB -
JKN67_RS01735 (370442) 370442..370915 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 356540..366027 9487


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10165.92 Da        Isoelectric Point: 10.7076

>T204863 WP_001259386.1 NZ_CP076237:365396-365671 [Escherichia coli O157:H7]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKLILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T204863 NZ_CP101269:c4602827-4602516 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT204863 WP_000593555.1 NZ_CP076237:365668-366027 [Escherichia coli O157:H7]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT204863 NZ_CP101269:c4602515-4602225 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I9WXY7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References