Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4953726..4954223 Replicon chromosome
Accession NZ_CP076235
Organism Escherichia coli O157:H7 strain 9.1_Anguil

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JKN68_RS24795 Protein ID WP_001173343.1
Coordinates 4953726..4953899 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JKN68_RS24800 Protein ID WP_000398619.1
Coordinates 4953927..4954223 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKN68_RS24785 (4950396) 4950396..4951853 + 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -
JKN68_RS24790 (4952090) 4952090..4953607 + 1518 WP_001295074.1 lysine--tRNA ligase -
JKN68_RS24795 (4953726) 4953726..4953899 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JKN68_RS24800 (4953927) 4953927..4954223 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JKN68_RS24805 (4954450) 4954450..4954722 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
JKN68_RS24810 (4954734) 4954734..4954964 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
JKN68_RS24815 (4955145) 4955145..4956776 + 1632 WP_001216477.1 sensor histidine kinase -
JKN68_RS24820 (4956773) 4956773..4957492 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T204860 WP_001173343.1 NZ_CP076235:c4953899-4953726 [Escherichia coli O157:H7]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T204860 NZ_CP101269:c4114702-4114325 [Escherichia coli]
ATGAACACATTACCCGATACACACGTACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACTCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT204860 WP_000398619.1 NZ_CP076235:c4954223-4953927 [Escherichia coli O157:H7]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT204860 NZ_CP101269:c4115160-4114792 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References