Detailed information of TA system TA204856

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4609028..4609578
• Replicon: chromosome
• Accession: NZ_CP076235
• Organism: Escherichia coli O157:H7 strain 9.1_Anguil

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: JKN68_RS23250
• Protein ID: WP_001160975.1
• Coordinates: 4609028..4609342 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: JKN68_RS23255
• Protein ID: WP_000125566.1
• Coordinates: 4609345..4609578 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKN68_RS23225 (4604703)	4604703..4605989	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
JKN68_RS23230 (4605989)	4605989..4606978	+	990	WP_000241259.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
JKN68_RS23235 (4606975)	4606975..4607796	+	822	WP_001065380.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
JKN68_RS23240 (4607799)	4607799..4608176	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
JKN68_RS23245 (4608183)	4608183..4609031	+	849	WP_000257187.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
JKN68_RS23250 (4609028)	4609028..4609342	-	315	WP_001160975.1	CcdB family protein	Toxin
JKN68_RS23255 (4609345)	4609345..4609578	-	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
JKN68_RS23260 (4609664)	4609664..4610143	-	480	WP_000624372.1	type 3 dihydrofolate reductase	-
JKN68_RS23265 (4610335)	4610335..4612197	-	1863	WP_000377184.1	glutathione-regulated potassium-efflux system protein KefC	-
JKN68_RS23270 (4612190)	4612190..4612720	-	531	WP_000600700.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
JKN68_RS23275 (4612828)	4612828..4614159	-	1332	WP_001183177.1	MFS transporter	-
JKN68_RS23280 (4614217)	4614217..4614504	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T204856 WP_001160975.1 NZ_CP076235:c4609342-4609028 [Escherichia coli O157:H7]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T204856 NZ_CP101269:3530662-3530880 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT204856 WP_000125566.1 NZ_CP076235:c4609578-4609345 [Escherichia coli O157:H7]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT204856 NZ_CP101269:3530262-3530636 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9