Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4113736..4114354 Replicon chromosome
Accession NZ_CP076235
Organism Escherichia coli O157:H7 strain 9.1_Anguil

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JKN68_RS20930 Protein ID WP_001291435.1
Coordinates 4114136..4114354 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JKN68_RS20925 Protein ID WP_000344800.1
Coordinates 4113736..4114110 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKN68_RS20915 (4108825) 4108825..4110018 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JKN68_RS20920 (4110041) 4110041..4113190 + 3150 WP_226168984.1 efflux RND transporter permease AcrB -
JKN68_RS20925 (4113736) 4113736..4114110 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JKN68_RS20930 (4114136) 4114136..4114354 + 219 WP_001291435.1 HHA domain-containing protein Toxin
JKN68_RS20935 (4114526) 4114526..4115077 + 552 WP_000102553.1 maltose O-acetyltransferase -
JKN68_RS20940 (4115193) 4115193..4115663 + 471 WP_000136192.1 YlaC family protein -
JKN68_RS20945 (4115827) 4115827..4117377 + 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JKN68_RS20950 (4117419) 4117419..4117772 - 354 WP_000878140.1 DUF1428 domain-containing protein -
JKN68_RS20960 (4118151) 4118151..4118462 + 312 WP_000409911.1 MGMT family protein -
JKN68_RS20965 (4118495) 4118495..4119067 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T204854 WP_001291435.1 NZ_CP076235:4114136-4114354 [Escherichia coli O157:H7]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T204854 NZ_CP101269:2754824-2754931 [Escherichia coli]
ATGACGCTCGCGAAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT204854 WP_000344800.1 NZ_CP076235:4113736-4114110 [Escherichia coli O157:H7]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT204854 NZ_CP101269:c2754771-2754710 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References