Detailed information of TA system TA204852

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 3491510..3491988
• Replicon: chromosome
• Accession: NZ_CP076235
• Organism: Escherichia coli O157:H7 strain 9.1_Anguil

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: JKN68_RS18075
• Protein ID: WP_001303876.1
• Coordinates: 3491701..3491988 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: JKN68_RS18070
• Protein ID: WP_000536233.1
• Coordinates: 3491510..3491701 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKN68_RS18035 (3486914)	3486914..3487684	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
JKN68_RS18040 (3487706)	3487706..3488452	-	747	WP_000788745.1	ATP-binding protein	-
JKN68_RS18045 (3488459)	3488459..3489550	-	1092	WP_001205823.1	hypothetical protein	-
JKN68_RS18050 (3489629)	3489629..3490084	+	456	WP_000273724.1	hypothetical protein	-
JKN68_RS18055 (3490291)	3490291..3490716	-	426	WP_000693855.1	toxin YdaT family protein	-
JKN68_RS18060 (3490700)	3490700..3490972	-	273	WP_000887453.1	YdaS family helix-turn-helix protein	-
JKN68_RS18065 (3491081)	3491081..3491482	+	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
JKN68_RS18070 (3491510)	3491510..3491701	+	192	WP_000536233.1	hypothetical protein	Antitoxin
JKN68_RS18075 (3491701)	3491701..3491988	+	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JKN68_RS18080 (3492266)	3492266..3492421	+	156	WP_000379575.1	DUF1391 family protein	-
JKN68_RS27505 (3492423)	3492423..3492551	+	129	WP_000344963.1	protein YdfB	-
JKN68_RS18085 (3492563)	3492563..3492952	+	390	WP_000394511.1	hypothetical protein	-
JKN68_RS18090 (3493139)	3493139..3493324	-	186	WP_001133046.1	hypothetical protein	-
JKN68_RS18095 (3493898)	3493898..3494086	+	189	WP_000413705.1	cell division inhibition protein DicB	-
JKN68_RS18100 (3494083)	3494083..3494274	+	192	WP_001098307.1	DUF1482 family protein	-
JKN68_RS18105 (3494368)	3494368..3496839	+	2472	WP_000034474.1	exonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T204852 WP_001303876.1 NZ_CP076235:3491701-3491988 [Escherichia coli O157:H7]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T204852 NZ_CP101269:c2509534-2509358 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT204852 WP_000536233.1 NZ_CP076235:3491510-3491701 [Escherichia coli O157:H7]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT204852 NZ_CP101269:c2509312-2508896 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA