Detailed information of TA system TA204848

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2766590..2767228
• Replicon: chromosome
• Accession: NZ_CP076235
• Organism: Escherichia coli O157:H7 strain 9.1_Anguil

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: JKN68_RS14050
• Protein ID: WP_000813794.1
• Coordinates: 2767052..2767228 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JKN68_RS14045
• Protein ID: WP_001270286.1
• Coordinates: 2766590..2767006 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKN68_RS14025 (2761742)	2761742..2762683	-	942	WP_001251315.1	ABC transporter permease	-
JKN68_RS14030 (2762684)	2762684..2763697	-	1014	WP_000220402.1	ABC transporter ATP-binding protein	-
JKN68_RS14035 (2763715)	2763715..2764860	-	1146	WP_000047432.1	ABC transporter substrate-binding protein	-
JKN68_RS14040 (2765105)	2765105..2766511	-	1407	WP_000760654.1	PLP-dependent aminotransferase family protein	-
JKN68_RS14045 (2766590)	2766590..2767006	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
JKN68_RS14050 (2767052)	2767052..2767228	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
JKN68_RS14055 (2767450)	2767450..2767680	+	231	WP_000494244.1	YncJ family protein	-
JKN68_RS14060 (2767772)	2767772..2769733	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
JKN68_RS14065 (2769806)	2769806..2770342	-	537	WP_000429145.1	DNA-binding transcriptional regulator SutR	-
JKN68_RS14070 (2770434)	2770434..2771607	+	1174	Protein_2748	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T204848 WP_000813794.1 NZ_CP076235:c2767228-2767052 [Escherichia coli O157:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T204848 NZ_CP101269:1849032-1849283 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT204848 WP_001270286.1 NZ_CP076235:c2767006-2766590 [Escherichia coli O157:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT204848 NZ_CP101269:1849280-1849534 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• PDB: 6HPB