Detailed information of TA system TA020484

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpA-brnA/BrnT_toxin-BrnA
• Location: 2289771..2290302
• Replicon: chromosome
• Accession: NC_002927
• Organism: Bordetella bronchiseptica RB50

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: K0MH61
• Locus tag: AYT36_RS10920
• Protein ID: WP_003812626.1
• Coordinates: 2290036..2290302 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: K0MF02
• Locus tag: AYT36_RS10915
• Protein ID: WP_003816743.1
• Coordinates: 2289771..2290049 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYT36_RS10890	2285194..2286288	+	1095	WP_010926390.1	TRAP transporter substrate-binding protein	-
AYT36_RS10895	2286500..2287129	+	630	WP_003812635.1	TRAP transporter small permease subunit	-
AYT36_RS10900	2287132..2288799	+	1668	WP_003812634.1	TRAP transporter large permease subunit	-
AYT36_RS10905	2288814..2289470	+	657	WP_010926391.1	phosphoribosylanthranilate isomerase	-
AYT36_RS10915	2289771..2290049	-	279	WP_003816743.1	BrnA antitoxin family protein	Antitoxin
AYT36_RS10920	2290036..2290302	-	267	WP_003812626.1	BrnT family toxin	Toxin
AYT36_RS10925	2290533..2292074	-	1542	WP_010926392.1	M81 family metallopeptidase	-
AYT36_RS10930	2292136..2293014	-	879	WP_010926393.1	tripartite tricarboxylate transporter substrate binding protein	-
AYT36_RS10935	2293411..2294043	+	633	WP_003812619.1	PAS domain-containing protein	-
AYT36_RS10940	2294154..2294864	+	711	WP_003812617.1	aquaporin Z	-
AYT36_RS10945	2294903..2295175	-	273	WP_003812615.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (12-86)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10037.47 Da
• Isoelectric Point: 7.9923

>T20484 WP_003812626.1 NC_002927:c2290302-2290036 [Bordetella bronchiseptica RB50]
MDITYDPAKNDKNIADRGLSFELVCGFEWASALVVEDTRQVYAETRYQALGKIDGRLHMVVFTVRGESLRIISLRKANAR
EVARYEKA

Nucleotide

• Length: 267 bp

>T20484 NC_002927:c2290302-2290036 [Bordetella bronchiseptica RB50]
ATGGATATCACCTACGACCCCGCCAAGAACGACAAGAACATTGCCGATCGCGGCCTGTCGTTCGAGTTGGTGTGTGGCTT
CGAGTGGGCCAGCGCGCTGGTGGTTGAGGATACCCGCCAGGTCTACGCCGAAACGCGCTATCAGGCGCTGGGCAAAATCG
ATGGCCGGCTCCATATGGTGGTATTCACGGTGCGCGGCGAGTCGCTGCGCATCATCAGCCTGCGTAAGGCAAATGCTCGT
GAGGTAGCTCGTTATGAAAAAGCCTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10412.89 Da
• Isoelectric Point: 10.5548

>AT20484 WP_003816743.1 NC_002927:c2290049-2289771 [Bordetella bronchiseptica RB50]
MKKPDPEKIDRDNPEWTDAEFRRAKPAAEVLPESVHAMLGIRRRGPQKTPTKQVVTIRLSPDVVEAFKASGTGWQTRVDA
ALRDWLKTHQPG

Nucleotide

• Length: 279 bp

>AT20484 NC_002927:c2290049-2289771 [Bordetella bronchiseptica RB50]
ATGAAAAAGCCTGACCCCGAAAAGATTGACCGCGACAACCCCGAATGGACGGACGCGGAATTTCGGCGGGCCAAGCCGGC
CGCCGAAGTACTGCCCGAGTCGGTGCACGCGATGCTCGGCATTCGTCGCCGTGGGCCTCAAAAGACACCCACCAAACAGG
TCGTCACCATCCGGCTATCGCCTGACGTGGTAGAAGCCTTCAAGGCCTCCGGCACCGGTTGGCAGACTCGCGTGGATGCA
GCCCTGCGGGATTGGCTGAAGACGCACCAGCCTGGCTAG

Structures

Toxin

• AlphaFold DB: K0MH61

Antitoxin

• AlphaFold DB: K0MF02