Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 401795..402595 Replicon chromosome
Accession NZ_CP076235
Organism Escherichia coli O157:H7 strain 9.1_Anguil

Toxin (Protein)


Gene name ataT Uniprot ID A0A891SNC1
Locus tag JKN68_RS01925 Protein ID WP_000342448.1
Coordinates 402068..402595 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag JKN68_RS01920 Protein ID WP_001277108.1
Coordinates 401795..402061 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKN68_RS01900 (397453) 397453..398121 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
JKN68_RS01905 (398114) 398114..399172 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
JKN68_RS01910 (399417) 399417..400271 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
JKN68_RS01915 (400542) 400542..401645 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
JKN68_RS01920 (401795) 401795..402061 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
JKN68_RS01925 (402068) 402068..402595 + 528 WP_000342448.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
JKN68_RS01930 (402592) 402592..402975 - 384 WP_000778769.1 aspartate 1-decarboxylase autocleavage activator PanM -
JKN68_RS01935 (403399) 403399..404508 + 1110 WP_001301528.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
JKN68_RS01940 (404556) 404556..405482 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
JKN68_RS01945 (405479) 405479..406756 + 1278 WP_000803797.1 branched chain amino acid ABC transporter permease LivM -
JKN68_RS01950 (406753) 406753..407520 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19692.68 Da        Isoelectric Point: 7.3232

>T204831 WP_000342448.1 NZ_CP076235:402068-402595 [Escherichia coli O157:H7]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T204831 NZ_CP101263:2380555-2381862 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT204831 WP_001277108.1 NZ_CP076235:401795-402061 [Escherichia coli O157:H7]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT204831 NZ_CP101263:2380289-2380555 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6GTS

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References