Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 359364..359995 Replicon chromosome
Accession NZ_CP076235
Organism Escherichia coli O157:H7 strain 9.1_Anguil

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag JKN68_RS01675 Protein ID WP_001259385.1
Coordinates 359364..359639 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag JKN68_RS01680 Protein ID WP_000593555.1
Coordinates 359636..359995 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKN68_RS01660 (354368) 354368..355435 + 1068 WP_000361458.1 HlyD family secretion protein -
JKN68_RS01665 (355432) 355432..358167 + 2736 WP_000149110.1 ribosome-associated ATPase/putative transporter RbbA -
JKN68_RS01670 (358167) 358167..359291 + 1125 WP_001301659.1 ABC transporter permease -
JKN68_RS01675 (359364) 359364..359639 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
JKN68_RS01680 (359636) 359636..359995 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
JKN68_RS01685 (360045) 360045..360905 - 861 WP_001131788.1 class II aldolase -
JKN68_RS01690 (360937) 360937..361206 - 270 WP_000933060.1 HPr family phosphocarrier protein -
JKN68_RS01695 (361196) 361196..362704 - 1509 WP_001301773.1 FGGY-family carbohydrate kinase -
JKN68_RS01700 (362697) 362697..364055 - 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
JKN68_RS01705 (364132) 364132..364413 - 282 WP_000084025.1 PTS sugar transporter subunit IIB -
JKN68_RS01710 (364410) 364410..364883 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 350508..359995 9487


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T204830 WP_001259385.1 NZ_CP076235:359364-359639 [Escherichia coli O157:H7]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T204830 NZ_CP101263:2038478-2038581 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT204830 WP_000593555.1 NZ_CP076235:359636-359995 [Escherichia coli O157:H7]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT204830 NZ_CP101263:c2038619-2038348 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References