Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | TsxAB/Xre(antitoxin) |
| Location | 9473..9974 | Replicon | plasmid p55 |
| Accession | NZ_CP076233 | ||
| Organism | Escherichia coli O157:H7 strain 7.1_Anguil | ||
Toxin (Protein)
| Gene name | TsxA | Uniprot ID | A0A2S5RIK4 |
| Locus tag | XE90_RS28290 | Protein ID | WP_022645141.1 |
| Coordinates | 9714..9974 (-) | Length | 87 a.a. |
Antitoxin (Protein)
| Gene name | TsxB | Uniprot ID | A0A2S5RIE4 |
| Locus tag | XE90_RS28285 | Protein ID | WP_022645142.1 |
| Coordinates | 9473..9724 (-) | Length | 84 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| XE90_RS28245 (XE90_028060) | 4833..5432 | + | 600 | WP_000091423.1 | hypothetical protein | - |
| XE90_RS28250 | 5452..5574 | + | 123 | Protein_5 | hypothetical protein | - |
| XE90_RS28255 (XE90_028070) | 5909..6087 | + | 179 | Protein_6 | hypothetical protein | - |
| XE90_RS28260 (XE90_028075) | 6421..6741 | + | 321 | WP_046424360.1 | hypothetical protein | - |
| XE90_RS28265 (XE90_028080) | 6873..7310 | - | 438 | WP_000466318.1 | type II toxin-antitoxin system HicB family antitoxin | - |
| XE90_RS28270 (XE90_028085) | 7335..7517 | - | 183 | WP_000833470.1 | type II toxin-antitoxin system HicA family toxin | - |
| XE90_RS28275 | 7814..8257 | + | 444 | WP_226326713.1 | hypothetical protein | - |
| XE90_RS28280 (XE90_028095) | 8645..8923 | - | 279 | WP_000545937.1 | hypothetical protein | - |
| XE90_RS28285 (XE90_028100) | 9473..9724 | - | 252 | WP_022645142.1 | transcriptional regulator | Antitoxin |
| XE90_RS28290 (XE90_028105) | 9714..9974 | - | 261 | WP_022645141.1 | hypothetical protein | Toxin |
| XE90_RS28295 (XE90_028110) | 10026..10250 | - | 225 | WP_000814383.1 | hypothetical protein | - |
| XE90_RS28300 (XE90_028115) | 10348..10557 | - | 210 | WP_000866037.1 | hypothetical protein | - |
| XE90_RS28305 (XE90_028120) | 10597..10920 | - | 324 | WP_000427402.1 | hypothetical protein | - |
| XE90_RS28310 (XE90_028125) | 11499..11828 | - | 330 | WP_000855121.1 | hypothetical protein | - |
| XE90_RS28315 (XE90_028130) | 12425..13090 | + | 666 | WP_000532148.1 | division plane positioning ATPase MipZ | - |
| XE90_RS28320 (XE90_028135) | 13164..13448 | + | 285 | WP_001162371.1 | hypothetical protein | - |
| XE90_RS28325 (XE90_028140) | 13466..14060 | - | 595 | Protein_20 | ProQ/FINO family protein | - |
| XE90_RS28330 (XE90_028145) | 14210..14329 | - | 120 | WP_227525580.1 | Hok/Gef family protein | - |
| XE90_RS28335 | 14277..14429 | - | 153 | Protein_22 | FlmC family protein | - |
| XE90_RS28340 (XE90_028150) | 14565..14726 | - | 162 | WP_001128311.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | - | 1..55027 | 55027 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 87 a.a. Molecular weight: 9876.23 Da Isoelectric Point: 7.2030
>T204826 WP_022645141.1 NZ_CP076233:c9974-9714 [Escherichia coli O157:H7]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEEAFDTIDFASDGLRENDKEIVSMMIQEMS
EEKNAN
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEEAFDTIDFASDGLRENDKEIVSMMIQEMS
EEKNAN
Download Length: 261 bp
>T204826 NZ_CP101263:888825-889232 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA
Antitoxin
Download Length: 84 a.a. Molecular weight: 9407.81 Da Isoelectric Point: 9.8120
>AT204826 WP_022645142.1 NZ_CP076233:c9724-9473 [Escherichia coli O157:H7]
MLTENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDS
GSN
MLTENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDS
GSN
Download Length: 252 bp
>AT204826 NZ_CP101263:888578-888844 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2S5RIK4 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2S5RIE4 |