Detailed information of TA system TA204823

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 5333164..5334001
• Replicon: chromosome
• Accession: NZ_CP076232
• Organism: Escherichia coli O157:H7 strain 7.1_Anguil

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7U9LL67
• Locus tag: XE90_RS26895
• Protein ID: WP_000854687.1
• Coordinates: 5333164..5333544 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: XE90_RS26900
• Protein ID: WP_012816826.1
• Coordinates: 5333633..5334001 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XE90_RS26865 (5328806)	5328806..5330647	+	1842	WP_000437371.1	RNA polymerase sigma factor RpoD	-
XE90_RS26870 (5330726)	5330726..5331232	-	507	WP_000228926.1	G/U mismatch-specific DNA glycosylase	-
XE90_RS26880 (5331532)	5331532..5332377	-	846	WP_001274558.1	DUF4942 domain-containing protein	-
XE90_RS26885 (5332462)	5332462..5332659	-	198	WP_000772026.1	DUF957 domain-containing protein	-
XE90_RS26890 (5332679)	5332679..5333167	-	489	WP_001054227.1	DUF5983 family protein	-
XE90_RS26895 (5333164)	5333164..5333544	-	381	WP_000854687.1	TA system toxin CbtA family protein	Toxin
XE90_RS26900 (5333633)	5333633..5334001	-	369	WP_012816826.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
XE90_RS26905 (5334076)	5334076..5334297	-	222	WP_000692340.1	DUF987 domain-containing protein	-
XE90_RS26910 (5334366)	5334366..5334842	-	477	WP_001364966.1	RadC family protein	-
XE90_RS26915 (5334858)	5334858..5335331	-	474	WP_001364892.1	antirestriction protein	-
XE90_RS26920 (5335425)	5335425..5335670	-	246	WP_000680588.1	hypothetical protein	-
XE90_RS26925 (5335670)	5335670..5336491	-	822	WP_046425418.1	DUF932 domain-containing protein	-
XE90_RS26930 (5336711)	5336711..5337121	-	411	WP_046425421.1	hypothetical protein	-
XE90_RS26935 (5337137)	5337137..5337814	-	678	WP_024226808.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14199.13 Da
• Isoelectric Point: 6.8615

>T204823 WP_000854687.1 NZ_CP076232:c5333544-5333164 [Escherichia coli O157:H7]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFSDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGVPSQLINSIDILRARRATGLMTRDNYRTVNNITLGRHPEEAKQ

Nucleotide

• Length: 381 bp

>T204823 NZ_CP101263:638980-639294 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13780.56 Da
• Isoelectric Point: 6.4763

>AT204823 WP_012816826.1 NZ_CP076232:c5334001-5333633 [Escherichia coli O157:H7]
VSDTLHETNYPNDNHNRPWWGLPCTVTPCFGARLVQEGNRLHYLDDRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVALYAKGLTCDADTLGSCGYVYLAVYPTPDMKK

Nucleotide

• Length: 369 bp

>AT204823 NZ_CP101263:639291-639707 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LL67