Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 5154793..5155447 Replicon chromosome
Accession NZ_CP076232
Organism Escherichia coli O157:H7 strain 7.1_Anguil

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag XE90_RS25970 Protein ID WP_000244781.1
Coordinates 5154793..5155200 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag XE90_RS25975 Protein ID WP_000354046.1
Coordinates 5155181..5155447 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
XE90_RS25950 (5150750) 5150750..5152483 - 1734 WP_000813185.1 single-stranded-DNA-specific exonuclease RecJ -
XE90_RS25955 (5152489) 5152489..5153199 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
XE90_RS25960 (5153224) 5153224..5154120 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
XE90_RS25965 (5154232) 5154232..5154753 + 522 WP_001055874.1 flavodoxin FldB -
XE90_RS25970 (5154793) 5154793..5155200 - 408 WP_000244781.1 protein YgfX Toxin
XE90_RS25975 (5155181) 5155181..5155447 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
XE90_RS25980 (5155690) 5155690..5156670 + 981 WP_000886053.1 tRNA-modifying protein YgfZ -
XE90_RS25985 (5156747) 5156747..5157406 - 660 WP_000250274.1 hemolysin III family protein -
XE90_RS25990 (5157570) 5157570..5157881 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
XE90_RS25995 (5157926) 5157926..5159359 + 1434 WP_001310226.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T204822 WP_000244781.1 NZ_CP076232:c5155200-5154793 [Escherichia coli O157:H7]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T204822 NZ_CP101263:c596210-595746 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT204822 WP_000354046.1 NZ_CP076232:c5155447-5155181 [Escherichia coli O157:H7]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT204822 NZ_CP101263:c596545-596210 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References